Kubo K, Ishikura T, Fukagawa Y
J Antibiot (Tokyo). 1984 Nov;37(11):1394-402. doi: 10.7164/antibiotics.37.1394.
A specific acylase designated A933 acylase was isolated and purified to 90% protein homogeneity from Streptomyces fulvoviridis A933 17M9 which produces PS-5, epithienamycins A and C and MM 17880 together with minor carbapenem analogs, penicillin N and cephamycin C. This enzyme was found to catalyze the depantothenylation of OA-6129 carbapenems; the acyl exchange of OA-6129 carbapenems with acyl CoA's; the deacetylation of N-acetyl-L-amino acids; and the acylation of NS-5 and 6-aminopenicillanate with acyl CoA's, whereas the deacetylation of PS-5 and N-acetyl-D-amino acids; and the deacylation of benzylpenicillin and cephalosporin C were not observed. Similar enzyme activities were also detected in Streptomyces cattleya, Streptomyces cremeus subsp. auratilis and Streptomyces argenteolus which are all carbapenem producers.
从黄绿链霉菌A933 17M9中分离并纯化出一种名为A933酰基转移酶的特异性酰基转移酶，其蛋白质纯度达到90%。黄绿链霉菌A933 17M9可产生PS-5、表硫霉素A和C以及MM 17880，同时还产生少量碳青霉烯类似物、青霉素N和头孢菌素C。发现该酶可催化OA-6129碳青霉烯类的去泛酰化反应；OA-6129碳青霉烯类与酰基辅酶A的酰基交换反应；N-乙酰-L-氨基酸的脱乙酰化反应；以及NS-5和6-氨基青霉烷酸与酰基辅酶A的酰化反应，而未观察到PS-5和N-乙酰-D-氨基酸的脱乙酰化反应；以及苄青霉素和头孢菌素C的脱酰化反应。在产碳青霉烯类的卡特利链霉菌、金色链霉菌亚种和银色链霉菌中也检测到了类似的酶活性。
