Lee T, Hastilow C, Smith K
Ross Laboratories, Analytical Research and Services, Columbus, OH 43216.
J Assoc Off Anal Chem. 1988 Jul-Aug;71(4):785-8.
A method has been developed for derivatization of monoglycerides and diglycerides to their propyl esters by using propionic anhydride in the presence of pyridine. The derivatives are volatile and thus can be separated and identified by gas chromatography (GC). Completion of the reaction is monitored by thin-layer chromatography. Monoglyceride propyl esters were positively identified by gas chromatography and by gas chromatography/mass spectrometry. No side products were observed in the derivatization reaction which requires 35 min at 75 degrees C. GC analysis of the mono- and diglyceride propyl esters can be completed in approximately 30 min.
已开发出一种方法，可在吡啶存在下使用丙酸酐将甘油单酯和甘油二酯衍生化为它们的丙酯。这些衍生物具有挥发性，因此可通过气相色谱法（GC）进行分离和鉴定。反应的完成情况通过薄层色谱法进行监测。甘油单酯丙酯通过气相色谱法和气相色谱/质谱法得到了确证。在75℃下需要35分钟的衍生化反应中未观察到副产物。甘油单酯和甘油二酯丙酯的GC分析大约可在30分钟内完成。
