Suman Sonal
Research Scholar Department of Biotechnology, Magadh University, Bodh Gaya, BR, India.
TPS College, Patna, BR, India.
Microbiol Insights. 2021 Jun 14;14:11786361211024289. doi: 10.1177/11786361211024289. eCollection 2021.
DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.
滴滴涕是所有使用过的不同类型有机氯农药中最持久的农药之一。在所有降解方法中，滴滴涕的细菌降解最为有效。本研究旨在从废物样本中分离出能够在尽可能短的时间内降解土壤中滴滴涕的不同细菌，并观察土壤样本不同物理和化学性质的影响。通过培养、生化试验分离并鉴定了许多农药降解细菌，并通过16S RNA测序方法进一步鉴定。最有效的菌株DDT 1在以滴滴涕作为唯一碳源（5 - 100 ppm）的矿物盐培养基中生长，并在600 nm光密度下进行监测。进一步优化了不同理化条件下的生长参数。结果表明，该菌株在15天内生长量最大。对培养15天后残留滴滴涕的傅里叶变换红外光谱分析表明，该菌株能够将农药降解为其进一步的代谢产物滴滴滴、滴滴伊、滴滴努等，可用于污染土壤和水生态系统中滴滴涕的生物降解。
