Baldassarre Hernan, Bordignon Vilceu
Department of Animal Science, McGill University, Quebec, Canada.
Anim Reprod. 2018 Aug 16;15(3):191-196. doi: 10.21451/1984-3143-AR2018-0057.
Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.
对2至6月龄的牛犊和水牛犊进行腹腔镜采卵（LOPU），随后进行胚胎生产并移植到同期成年受体体内，这是加速遗传进展和顶级遗传基因早期传播的有力工具。就目前的技术水平而言，与成年动物相比，该技术具有较高的卵母细胞回收率、较低的卵母细胞到胚胎转化率以及相似的妊娠和足月发育率。近年来，主要通过为青春期前供体量身定制的促性腺激素刺激方案，卵母细胞的质量得到了改善。这些进展使该技术达到了适合商业应用的程度。然而，未来的研究必须专注于提高从犊牛中回收的完全成熟卵母细胞的比例，从而进一步增强该技术平台在加速优良遗传基因传播计划中所能发挥的作用。
