Sanches Bruno Valente, Zangirolamo Amanda Fonseca, Seneda Marcelo Marcondes
Vytelle IVF, LLC, Hermiston, OR, USA.
Universidade Estadual de Londrina, Laboratório de Reprodução Animal, DCV-CCA-UEL, Londrina, Parana, Brazil.
Anim Reprod. 2019 Oct 23;16(3):394-401. doi: 10.21451/1984-3143-AR2019-0058.
The number of embryos produced by fertilization (IVF) has grown exponentially in recent years. Recently, for the first time, the number of embryos produced and transferred was significantly higher than the number developed worldwide. In this context, a particular boost occurred with ovum pick-up (OPU) and embryos produced in North America, and this technology is becoming more prominent for commercial dairy farms. However, despite many advances in recent decades, laboratories and companies are looking for methods and alternatives that can be used in collaboration with the existing process to improve it. Among the strategies used to improve the dairy industry are the use of genomic analysis for the selection of animals with desired traits or as an evaluation tool of oocyte and embryo quality, the optimization of the collection and use of gametes from prepubertal females and males, the effective use of sexed semen, and improvements in culture media and methods of embryo cryopreservation. Thus, this review aims to discuss the highlights of the commercial use of IVF and some strategies to increase the application of this technique in large-scale dairy programs.
近年来，通过体外受精（IVF）产生的胚胎数量呈指数增长。最近，首次出现全球范围内产生并移植的胚胎数量显著高于发育的胚胎数量。在这种背景下，北美通过采卵（OPU）产生的胚胎数量有了特别的增长，这项技术在商业化奶牛场中变得越来越突出。然而，尽管近几十年来取得了许多进展，实验室和公司仍在寻找可与现有流程协作使用的方法和替代方案来加以改进。用于改善奶牛养殖业的策略包括利用基因组分析来选择具有所需性状的动物，或将其作为卵母细胞和胚胎质量的评估工具，优化从青春期前雌性和雄性动物采集和使用配子，有效使用性别分选精液，以及改进培养基和胚胎冷冻保存方法。因此，本综述旨在讨论体外受精商业化应用的要点以及一些在大规模奶牛项目中增加该技术应用的策略。
