Zangirolamo Amanda Fonseca, Morotti Fabio, da Silva Nathalia Covre, Sanches Tamires Korchovei, Seneda Marcelo M
Universidade Estadual de Londrina, Laboratório de Reprodução Animal (DCV-CCA-UEL), Londrina, PR, Brazil.
National Institute of Science and Technology for Dairy Production Chain (INCT-LEITE), Universidade Estadual de Londrina, Londrina, PR, Brazil.
Anim Reprod. 2018 Aug 17;15(3):310-315. doi: 10.21451/1984-3143-AR2018-0072.
Reproductive biotechniques such as embryo production are important tools to increase the reproductive performance in cattle in a short time. In this context, the antral follicle count (AFC), which reflects the population of antral follicles present in an ovary, has been indicated as an important phenotypic characteristic related to female fertility and closely correlated to the performance of and embryo production (IVEP). A positive correlation was evidenced between AFC and oocyte retrieval by ovum pick up (OPU) sessions and and with the number of embryos produced. Several studies have reported that females with a high AFC had greater embryo yields compared to those with medium and low AFC. However, controversial results were obtained by studies conducted in different bovine breeds. Many conflicting data may be due to the differences in the experimental design, particularly regarding the classification of animals in AFC groups, subspecies particularities, herd aptitude or even issues related to animal management. Therefore, aspects such as the choice of donor, type of aspirated follicles and the stage of follicular wave need to be clarified. Thus, this text aims to discuss the use of AFC as a reproductive tool and its applications in the and production of embryos, besides describing consistent results and new challenges regarding AFC and embryo production.
胚胎生产等生殖生物技术是短期内提高奶牛繁殖性能的重要工具。在此背景下,反映卵巢中存在的腔前卵泡数量的腔前卵泡计数(AFC)已被表明是与雌性生育力相关的重要表型特征,并且与体外胚胎生产(IVEP)的性能密切相关。AFC与通过采卵(OPU)获得的卵母细胞数量以及产生的胚胎数量之间存在正相关。多项研究报告称,与AFC中等和较低的雌性相比,AFC高的雌性胚胎产量更高。然而,在不同牛品种中进行的研究得到了相互矛盾的结果。许多相互矛盾的数据可能是由于实验设计的差异,特别是关于动物在AFC组中的分类、亚种特殊性、牛群适应性甚至与动物管理相关的问题。因此,供体的选择、抽吸卵泡的类型和卵泡波的阶段等方面需要阐明。因此,本文旨在讨论AFC作为一种生殖工具的用途及其在胚胎体外生产中的应用,此外还描述了关于AFC和胚胎生产的一致结果和新挑战。