Li Zhenghua, Yang Xieliu, Zhang Qi, Yang Wenguang, Zhang Hemin, Liu Lianqing, Liang Wenfeng
School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang 110168, China.
School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China.
Biomicrofluidics. 2021 Jun 14;15(3):031501. doi: 10.1063/5.0052185. eCollection 2021 May.
The demand to understand the mechanical properties of cells from biomedical, bioengineering, and clinical diagnostic fields has given rise to a variety of research studies. In this context, how to use lab-on-a-chip devices to achieve accurate, high-throughput, and non-invasive acquisition of the mechanical properties of cells has become the focus of many studies. Accordingly, we present a comprehensive review of the development of the measurement of mechanical properties of cells using passive microfluidic mechanisms, including constriction channel-based, fluid-induced, and micropipette aspiration-based mechanisms. This review discusses how these mechanisms work to determine the mechanical properties of the cell as well as their advantages and disadvantages. A detailed discussion is also presented on a series of typical applications of these three mechanisms to measure the mechanical properties of cells. At the end of this article, the current challenges and future prospects of these mechanisms are demonstrated, which will help guide researchers who are interested to get into this area of research. Our conclusion is that these passive microfluidic mechanisms will offer more preferences for the development of lab-on-a-chip technologies and hold great potential for advancing biomedical and bioengineering research studies.
来自生物医学、生物工程和临床诊断领域对了解细胞力学特性的需求引发了各种研究。在此背景下，如何利用芯片实验室设备实现对细胞力学特性的准确、高通量和非侵入性获取已成为众多研究的焦点。因此，我们对利用被动微流控机制测量细胞力学特性的发展进行了全面综述，包括基于收缩通道、流体诱导和基于微吸管抽吸的机制。本综述讨论了这些机制如何工作以确定细胞的力学特性以及它们的优缺点。还对这三种机制测量细胞力学特性的一系列典型应用进行了详细讨论。在本文结尾，展示了这些机制当前面临的挑战和未来前景，这将有助于指导有兴趣进入该研究领域的研究人员。我们的结论是，这些被动微流控机制将为芯片实验室技术的发展提供更多优势，并在推进生物医学和生物工程研究方面具有巨大潜力。
