采用顶空气相色谱法测定大鼠肝脏匀浆中的三氯乙烯代谢物。

Determination of trichloroethylene metabolites in rat liver homogenate using headspace gas chromatography.

作者信息

Køppen B, Dalgaard L, Christensen J M

机构信息

Department of Chemistry BC, Royal Danish School of Pharmacy, Copenhagen.

出版信息

J Chromatogr. 1988 Jun 17;442:325-32. doi: 10.1016/s0021-9673(00)94480-7.

DOI:10.1016/s0021-9673(00)94480-7

PMID:3417823

Abstract

An headspace gas chromatographic (HSGC) method for determination of trichloroethylene metabolites in rat liver homogenates is described. These metabolites are chloral hydrate (CH), trichloroethanol (TCE), trichloroacetic acid (TCA) and the glucuronic acid conjugate of trichloroethanol (TCE-beta-glucuronide). The method is based on selective thermal conversion of CH and TCA into chloroform, which is determined together with trichloroethanol by HSGC using electron-capture detection. TCE-beta-glucuronide was determined as the difference between free TCE and total TCE after enzymatic hydrolysis with beta-glucuronidase. Synthesized TCE-beta-glucuronide was used to compare the efficiency of enzymatic and acid hydrolysis of the conjugate. Enzymatic hydrolysis was found to be advantageous for determination of TCE-beta-glucuronide.

摘要

描述了一种用于测定大鼠肝脏匀浆中三氯乙烯代谢物的顶空气相色谱（HSGC）方法。这些代谢物是水合氯醛（CH）、三氯乙醇（TCE）、三氯乙酸（TCA）和三氯乙醇的葡萄糖醛酸共轭物（TCE-β-葡萄糖醛酸苷）。该方法基于将CH和TCA选择性热转化为氯仿，氯仿与三氯乙醇一起通过使用电子捕获检测的HSGC进行测定。TCE-β-葡萄糖醛酸苷通过用β-葡萄糖醛酸酶进行酶水解后，以游离TCE与总TCE之间的差值来测定。合成的TCE-β-葡萄糖醛酸苷用于比较该共轭物的酶水解和酸水解效率。发现酶水解对于TCE-β-葡萄糖醛酸苷的测定是有利的。