Wang Haidong, Ba Yudong, Han Wenxiu, Zhang Haixia, Zhu Laiqing, Jiang Pei
Department of Pharmacy, The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, China.
Department of Pharmacy, Dongying People's Hospital, Dongying, China.
PeerJ. 2021 Jun 18;9:e11636. doi: 10.7717/peerj.11636. eCollection 2021.
Coronary artery disease (CAD) is one of the severe diseases that threaten human health worldwide. In addition, the associated rate of comorbidity with depression and anxiety is extremely high. Heat shock proteins (HSPs) are a group of proteins that possesses cardiovascular and psychological protection properties. The objective of this study is to determine the association of the two most widely studied HSPs, namely, HSP70 and HSP90, with CAD comorbid depression and anxiety in a Chinese population.
A case-control study involving 271 CAD patients and 113 healthy individuals was conducted. The 271 CAD patients include individuals with (123) and without depression (148) and individuals with (57) and without anxiety (214). Ten single nucleotide polymorphisms (SNPs) for HSP70 and seven SNPs for HSP90 were selected and genotyped.
Results revealed that the HSP70 rs10892958 C allele and HSP70 rs2236658 T allele were associated with a decreased risk of CAD ( < 0.05), whereas the G allele of the rs11218941 polymorphism was associated with an increased risk of CAD. The haplotype analysis results indicated that the haplotype TGGGC of the HSPA8 gene (coded the HSP70 family, rs4936770/rs4802/rs10892958/rs11218941/rs2236658) significantly increased the risk of CAD ( = 0.008). Among the patients with CAD, the carriers of the CC genotype for the HSP90 rs1042665 showed higher risks of anxiety than the carriers of another genotypes. However, no significant relationships were found among the CAD with depression and CAD without depression groups for the selected SNPs. These findings suggested that the genetic polymorphisms in the HSP gene, especially the HSPA8 of HSP70, contribute to CAD susceptibility and rs1042665 genetic polymorphisms might have an effect on the anxiety incidence among CAD patients.
冠状动脉疾病（CAD）是全球范围内威胁人类健康的严重疾病之一。此外，其与抑郁症和焦虑症的合并发生率极高。热休克蛋白（HSPs）是一类具有心血管和心理保护特性的蛋白质。本研究的目的是确定在中国人群中研究最广泛的两种热休克蛋白，即HSP70和HSP90，与CAD合并抑郁症和焦虑症之间的关联。
进行了一项病例对照研究，纳入271例CAD患者和113名健康个体。271例CAD患者包括伴有（123例）和不伴有抑郁症（148例）的个体，以及伴有（57例）和不伴有焦虑症（214例）的个体。选择了10个HSP70的单核苷酸多态性（SNPs）和7个HSP90的SNPs进行基因分型。
结果显示，HSP70 rs10892958 C等位基因和HSP70 rs2236658 T等位基因与CAD风险降低相关（<0.05），而rs11218941多态性的G等位基因与CAD风险增加相关。单倍型分析结果表明，HSPA8基因（编码HSP70家族，rs4936770/rs4802/rs10892958/rs11218941/rs2236658）的单倍型TGGGC显著增加了CAD风险（=0.008）。在CAD患者中，HSP90 rs1042665的CC基因型携带者比其他基因型携带者患焦虑症的风险更高。然而，在所选择的SNPs中，CAD伴抑郁症组和CAD不伴抑郁症组之间未发现显著关系。这些发现表明，HSP基因中的遗传多态性，尤其是HSP70的HSPA8，与CAD易感性有关，rs1042665遗传多态性可能对CAD患者的焦虑症发病率有影响。
