尼日利亚疟疾病媒（广义）中杀虫剂抗性的生化机制

Biochemical Mechanism of Insecticide Resistance in Malaria Vector, s.l in Nigeria.

作者信息

Yusuf Mustapha Ahmed, Vatandoost Hassan, Oshaghi Mohammad Ali, Hanafi-Bojd Ahmad Ali, Manu Abdulsalam Yayo, Enayati Ahmadali, Ado Abduljalal, Abdullahi Alhassan Sharrif, Jalo Rabiu Ibrahim, Firdausi Abubakar

机构信息

Department of Medical Entomology & Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, Kano, Nigeria.

出版信息

Iran J Public Health. 2021 Jan;50(1):101-110. doi: 10.18502/ijph.v50i1.5076.

DOI:10.18502/ijph.v50i1.5076

PMID:34178768

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8213627/

Abstract

BACKGROUND

Malaria is a parasitic vector-borne disease endemic in the tropical and subtropical countries of the world. The aim of this study was to investigate the current activities of the detoxification enzymes in resistant and susceptible s.l. in northern Nigeria.

METHODS

larvae were collected from northeast and northwestern Nigeria between Aug and Nov 2018. Biochemical analyses was carried out on the mosquitoes exposed to various insecticides (deltamethrin, DDT, bendiocarb, malathion) to measure and compare the enzymatic activities of the major detoxification enzymes (P, GSTs, Esterase).

RESULTS

High levels of resistance was observed; DDT 37%-53% (95%, CI: 29-61), bendiocarb 44%-55% (CI: 39-60) and deltamethrin 74%-82% (CI: 70-86). However, these mosquitoes were found to be susceptible to malathion 99%-100% (CI: 98-100). The P450 and GSTs enzymes were found to be elevated in the resistant mosquitoes exposed to deltamethrin (1.0240±0.1902); (1.3088±1.2478), DDT (1.7703±1.4528); (1.7462±0.9418) and bendiocarb (1.1814±0.0918); (1.4479±1.0083) compared to the Kisumu strain (0.764±0.4226); (0.6508±0.6542), (0.3875±0.3482); (0.4072±0.4916) and (0.6672±0.3949); (0.7126±0.7259) at <0.05. Similarly, the resistant mosquitoes expressed increased activity to esterase (0.7606±1.1477), (0.3269±1.1957) and (2.8203±0.6488) compared to their susceptible counterpart (0.6841±0.7597), (0.7032±0.5380) and (0.6398±0.4159) at <0.05. The enzyme ratio was found to be: P450 (1.341, 4.568 and 1.77); GSTs (2.011, 4.288 and 2.031); Esterases (1.111, 0.469 and 4.408). One way Anova and single sample t-test were also conducted to determine the effect of the enzymes on the resistant and susceptible strains.

CONCLUSION

High level of insecticide resistance was observed with significant elevation of detoxification enzymes activities in the resistant mosquitoes.

摘要

背景

疟疾是一种由寄生虫通过媒介传播的疾病，在世界热带和亚热带国家流行。本研究的目的是调查尼日利亚北部抗药和敏感按蚊中解毒酶的当前活性。

方法

2018年8月至11月间从尼日利亚东北部和西北部收集幼虫。对接触各种杀虫剂（溴氰菊酯、滴滴涕、残杀威、马拉硫磷）的蚊子进行生化分析，以测量和比较主要解毒酶（细胞色素P450、谷胱甘肽S-转移酶、酯酶）的酶活性。

结果

观察到高水平的抗性；滴滴涕为37%-53%（95%，置信区间：29-61），残杀威为44%-55%（置信区间：39-60），溴氰菊酯为74%-82%（置信区间：70-86）。然而，发现这些蚊子对马拉硫磷敏感，敏感度为99%-100%（置信区间：98-100）。与基苏木品系（0.764±0.4226）；（0.6508±0.6542）、（0.3875±0.3482）；（0.4072±0.4916）和（0.6672±0.3949）；（0.7126±0.7259）相比，在<0.05水平下，接触溴氰菊酯（1.0240±0.1902）；（1.3088±1.2478）、滴滴涕（1.7703±1.4528）；（1.7462±0.9418）和残杀威（1.1814±0.0918）；（1.4479±1.0083）的抗性蚊子中，细胞色素P450和谷胱甘肽S-转移酶酶升高。同样，与敏感蚊子（0.6841±0.7597）；（0.7032±0.5380）和（0.6398±0.4159）相比，在<0.05水平下，抗性蚊子对酯酶的活性增加（0.7606±1.1477）；（0.3269±1.1957）和（2.8203±0.6488）。酶比率为：细胞色素P450（1.341、4.568和1.77）；谷胱甘肽S-转移酶（2.011、4.288和2.031）；酯酶（1.111、0.469和4.408）。还进行了单因素方差分析和单样本t检验，以确定这些酶对抗性和敏感品系的影响。