Olatunbosun-Oduola Adedayo, Abba Ezra, Adelaja Olukayode, Taiwo-Ande Adeolu, Poloma-Yoriyo Kennedy, Samson-Awolola Taiwo
Department of Zoology, University of Ilorin, Ilorin, Kwara State, Nigeria.
Department of Biological Sciences, Faculty of Science, Gombe State University PMB 127, Gombe, Nigeria.
J Arthropod Borne Dis. 2019 Mar 30;13(1):50-61. eCollection 2019 Mar.
Timely entomological and insecticide resistance monitoring is a key to generating relevant data for vector management. We investigated the insecticide susceptibility status of s.l. in eight rural farming communities in Southern Gombe, Nigeria.
Overall, 3-5 days-old adult female mosquitoes reared from field-collected immature stages between September and November, 2014 were exposed to the diagnostic doses of pyrethroids, organophosphate and carbamate insecticides using the Center for Disease Control Bottle bioassay. The observatory knockdown time from exposure to each insecticide was recorded up to two hours. The dead mosquitoes were then identified morphologically and by molecular assays.
Mortality results showed resistance in s.l. populations to bendiocarb (2.3-100%), deltamethrin (39-70%), pirimiphos-methyl (65-95%), dichloro-diphenyl-trichloroethane (0-38.1%), permethrin (0-46.3%) and lambda-cyhalothrin (42.5-86.4%). The few cases of full susceptibility were observed from lamdacyhalothrin exposed population of s.l. in Banbam and Pantami respectively. 177 (45%) was significantly higher (P< 0.05) than 64 (16.3%), 34 (8.7%) and hybrid 78 (19.8%).
A strong evidence of widespread resistance in the major malaria vector species in Southern Gombe to all common classes of insecticides is a justification for the State Malaria Elimination Programme to consciously consider incorporating insecticide resistance management strategies into control programs in order to sustain the future of current control interventions.
及时进行昆虫学和杀虫剂抗性监测是生成病媒管理相关数据的关键。我们调查了尼日利亚贡贝南部八个农村农业社区中冈比亚按蚊复合体的杀虫剂敏感性状况。
总体而言，2014年9月至11月间从野外采集的未成熟阶段饲养的3至5日龄成年雌性冈比亚按蚊复合体蚊子，使用疾病控制中心瓶式生物测定法，接触拟除虫菊酯、有机磷和氨基甲酸酯类杀虫剂的诊断剂量。记录从接触每种杀虫剂到两小时内的观察击倒时间。然后对死亡蚊子进行形态学和分子检测鉴定。
死亡率结果显示，冈比亚按蚊复合体种群对残杀威（2.3% - 100%）、溴氰菊酯（39% - 70%）、甲基嘧啶磷（65% - 95%）、滴滴涕（0% - 38.1%）、氯菊酯（0% - 46.3%）和高效氯氟氰菊酯（42.5% - 86.4%）具有抗性。在班班和潘塔米分别观察到少数冈比亚按蚊复合体种群对高效氯氟氰菊酯完全敏感的情况。177（45%）显著高于64（16.3%）、34（8.7%）和杂交种78（19.8%）（P < 0.05）。
有强有力的证据表明，贡贝南部主要疟疾媒介物种对所有常见类别的杀虫剂普遍具有抗性，这证明该州疟疾消除计划有必要有意识地考虑将杀虫剂抗性管理策略纳入控制项目，以维持当前控制干预措施的未来成效。
