Yan Min, Wang Jing, Wang Suyun, Zhang Yang, Liu Ling, Zhao Hua
Department of Pediatrics, Jinan City People's Hospital Affiliated to Shandong First Medical University, Jinan 271199, China.
Department of Physical Examination, the People's Hospital of Zhangqiu Area, Jinan 250200, China.
Iran J Public Health. 2021 Mar;50(3):510-519. doi: 10.18502/ijph.v50i3.5592.
To investigate the expression levels of microRNA-146b (miR-146b) and cardiac troponin I (anti-cTnI) in serum of children with viral myocarditis and their clinical significance.
Forty-eight children with viral myocarditis (patient group) and 40 healthy physical examinees (healthy group), who were diagnosed in Jinan City People's Hospital Affiliated to Shandong First Medical University, China from Feb 2018 to May 2019, were enrolled as study subjects. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the level of miR-146b in serum of children. ELISA was used to detect the expression of anti-cTnI in serum of children. Pearson was used to analyze the correlation between the level of miR-146b and the level of anti-cTnI, and the factors affecting the prognosis.
The levels of miR-146b and anti-cTnI in serum of children in patient group were statistically significantly higher than those of healthy group (<0.01). The AUC of miR-146b was 0.741, (95% CI: 0.638-0.843), the specificity was 62.50%, the sensitivity was 82.50%, and the AUC of anti-cTnI was 0.720 (95% CI: 0.608-0.832), the specificity was 64.58% and the sensitivity was 92.50%. The level of miR-146b was positively correlated with the level of anti-cTnI (r=0.601, <0.05). CK-MB, LVEF, miR-146b and anti-cTnI expression were independent risk factors affecting the prognosis.
The levels of miR-146b and anti-cTnI increased in serum of patients with viral myocarditis. They were related to the degree of myocardial injury, which indicated that miR-146b and anti-cTnI might be involved in the pathological process of viral myocarditis.
探讨微小RNA-146b（miR-146b）和心肌肌钙蛋白I（抗cTnI）在病毒性心肌炎患儿血清中的表达水平及其临床意义。
选取2018年2月至2019年5月在山东第一医科大学附属济南市人民医院确诊的48例病毒性心肌炎患儿（患者组）和40例健康体检者（健康组）作为研究对象。采用逆转录聚合酶链反应（RT-PCR）检测患儿血清中miR-146b水平。采用酶联免疫吸附测定（ELISA）检测患儿血清中抗cTnI的表达。采用Pearson分析miR-146b水平与抗cTnI水平的相关性以及影响预后的因素。
患者组患儿血清中miR-146b和抗cTnI水平显著高于健康组（<0.01）。miR-146b的曲线下面积（AUC）为0.741（95%可信区间：0.638-0.843），特异性为62.50%，敏感性为82.50%；抗cTnI的AUC为0.720（95%可信区间：0.608-0.832），特异性为64.58%，敏感性为92.50%。miR-146b水平与抗cTnI水平呈正相关（r=0.601，<0.05）。肌酸激酶同工酶（CK-MB）、左心室射血分数（LVEF）、miR-146b和抗cTnI表达是影响预后的独立危险因素。
病毒性心肌炎患者血清中miR-146b和抗cTnI水平升高。它们与心肌损伤程度相关，提示miR-146b和抗cTnI可能参与病毒性心肌炎的病理过程。
