Li Yanping, Lu Bin, Yu Muming, Zhai Jianhua, Yao Ying, Chai Yanfen
Department of Emergency Medicine, Tianjin Medical University General Hospital, Tianjin 300052, P.R. China.
Exp Ther Med. 2021 Dec;22(6):1396. doi: 10.3892/etm.2021.10832. Epub 2021 Oct 1.
In previous studies, miR-132 and miR-223 were considered to be involved in cellular and pathological processes of diseases. However, the role of early diagnosis and prognosis evaluation in sepsis-induced cardiomyopathy (SIC) remains unknown. The present study aimed to explore the diagnostic value of combined detection of miR-132 and miR-223 for SIC and their correlation with creatine kinase-MB (CK-MB), cardiac troponin I (cTnI), tumor necrosis factor α (TNF-α), and interleukin-6 (IL)-6. SIC patients (n=80) admitted to Tianjin Medical University General Hospital were assigned to the research group (RG), while 60 healthy participants receiving physical examinations at the same period were assigned to the control group (CG). Serum expression profiles of miR-132 and miR-223 were detected by the RT-qPCR. CK-MB and cTnI were assessed using chemiluminescence assay, and TNF-α and IL-6 by enzyme-linked immunosorbent assay (ELISA). Serum miR-132 and miR-223 levels were significantly lower in the RG than in the CG (P<0.001). The sensitivity and specificity for the diagnosis of SIC were 82.50 and 71.67% for miR-132, 95.00 and 61.67% for miR-223, as well as 86.25 and 86.67% for miR-132 combined with miR-223. Serum miR-132 and miR-223 levels were significantly higher in the survivor group than in the deceased group (P<0.001). The sensitivity and specificity for the prognosis of SIC were 85.96 and 65.22% for miR-132 combined with miR-223. Serum miR-132 and miR-223 were negatively correlated with serum CK-MB, cTnI, TNF-α, and IL-6 (P<0.001). miR-132 combined with miR-223 can be used for early diagnosis and prognostic evaluation of SIC, and the two are correlated with CK-MB, cTnI, TNF-α, and IL-6.
在先前的研究中，miR - 132和miR - 223被认为参与了疾病的细胞和病理过程。然而，其在脓毒症诱导的心肌病（SIC）早期诊断和预后评估中的作用仍不清楚。本研究旨在探讨联合检测miR - 132和miR - 223对SIC的诊断价值及其与肌酸激酶同工酶（CK - MB）、心肌肌钙蛋白I（cTnI）、肿瘤坏死因子α（TNF - α）和白细胞介素 - 6（IL - 6）的相关性。将天津医科大学总医院收治的80例SIC患者纳入研究组（RG），同期60例接受体检的健康参与者纳入对照组（CG）。采用RT - qPCR检测血清中miR - 132和miR - 223的表达谱。采用化学发光法检测CK - MB和cTnI，采用酶联免疫吸附测定法（ELISA）检测TNF - α和IL - 6。研究组血清miR - 132和miR - 223水平显著低于对照组（P < 0.001）。miR - 132诊断SIC的灵敏度和特异度分别为82.50%和71.67%，miR - 223分别为95.00%和61.67%，miR - 132联合miR - 223分别为86.25%和86.67%。存活组血清miR - 132和miR - 223水平显著高于死亡组（P < 0.001）。miR - 132联合miR - 223对SIC预后评估的灵敏度和特异度分别为85.96%和65.22%。血清miR -
