Shokoohi Ebrahim, Abolafia Joaquín, Mashela Phatu William, Divsalar Nafiseh
Green Biotechnologies Research Centre of Excellence, University of Limpopo, Private Bag, X1106, Sovenga, 0727, South Africa.
Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus 'Las Lagunillas' s/n. 23071-Jaén, Spain.
J Nematol. 2019 Aug 5;51. doi: 10.21307/jofnem-2019-041. eCollection 2019.
from Iran and from South Africa were recovered during a survey of plant-parasitic nematodes belonging to the family Pratylenchidae. Both species were studied using morphological and molecular techniques. is identified based on the flattened head, short stylet (19-22 µm), excretory pore position (anterior to pharyngo-intestinal junction), spicule length (27-30 µm), and existence of an axial mucro at the tail end. Phylogenetic analysis using 28S rDNA showed monophyly of which Iranian placed close to (EU620464; EU620465). This result was supported by the principal component analysis of species. SEM observation of the South African population of showed the presence of two annuli in the lip region. Morphometric characters resembled those of specimens earlier reported from South Africa. Hierarchal cluster using morphometrical criteria showed that the Floridian (USA) and South African populations form a group. However, the principal component analysis showed variation within this species. The molecular study of populations using 18S, ITS, 28S rDNA, and COI of mtDNA showed that all cluster in one group and confirmed the identification of the species using both morphological and molecular techniques. In addition, the results indicated that South African populations group close to the USA populations. Illustrations of both species including light and scanning electron microscopy observations for are provided.
在一项对短体线虫科植物寄生线虫的调查中，从伊朗和南非采集到了[具体物种名称未给出]。使用形态学和分子技术对这两个物种进行了研究。[具体物种名称未给出]是根据扁平的头部、短口针（19 - 22微米）、排泄孔位置（咽-肠交界处前方）、交合刺长度（27 - 30微米）以及尾端存在轴向小刺来鉴定的。使用28S rDNA进行的系统发育分析表明[具体物种名称未给出]是单系的，其中伊朗的[具体物种名称未给出]与[相关物种名称未给出]（EU620464；EU620465）亲缘关系较近。这一结果得到了该物种主成分分析的支持。对南非[具体物种名称未给出]种群的扫描电子显微镜观察显示唇部区域有两个环纹。形态测量特征与先前报道的南非标本相似。使用形态测量标准进行的层次聚类表明，佛罗里达（美国）和南非种群形成一个组。然而，主成分分析显示该物种内部存在变异。使用18S、ITS、28S rDNA和线粒体DNA的COI对[具体物种名称未给出]种群进行的分子研究表明，所有[具体物种名称未给出]都聚类在一组，并用形态学和分子技术证实了该物种的鉴定。此外，结果表明南非种群与美国种群聚类较近。提供了这两个物种的图示，包括[具体物种名称未给出]的光学显微镜和扫描电子显微镜观察结果。
