Department of Chemical Engineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan 44610, Republic of Korea.
J Ind Microbiol Biotechnol. 2021 Aug 24;48(7-8). doi: 10.1093/jimb/kuab039.
We investigated the effect of cell-surface display of glutamate decarboxylase (GadB) on gamma-aminobutyric acid (GABA) production in recombinant Escherichia coli. We integrated GadB from the hyperthermophilic, anaerobic archaeon Pyrococcus horikoshii to the C-terminus of the E. coli outer membrane protein C (OmpC). After 12 hr of culturing GadB-displaying cells, the GABA concentration in the extracellular medium increased to 3.2 g/l, which is eight times that obtained with cells expressing GadB in the cytosol. To further enhance GABA production, we increased the temperatures of the culture. At 60°C, the obtained GABA concentration was 4.62 g/l after 12 hr of culture, and 5.35 g/l after 24 hr, which corresponds to a yield of 87.7%.
我们研究了谷氨酸脱羧酶（GadB）在重组大肠埃希菌中细胞表面展示对γ-氨基丁酸（GABA）生产的影响。我们将来自嗜热、厌氧古菌 Pyrococcus horikoshii 的 GadB 整合到大肠埃希菌外膜蛋白 C（OmpC）的 C 末端。培养 GadB 展示细胞 12 小时后，细胞外培养基中 GABA 的浓度增加到 3.2 g/l，是在细胞质中表达 GadB 的细胞获得的 GABA 浓度的 8 倍。为了进一步提高 GABA 的产量，我们提高了培养温度。在 60°C 下，培养 12 小时后获得的 GABA 浓度为 4.62 g/l，培养 24 小时后为 5.35 g/l，对应得率为 87.7%。
