Division of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, Stockholm, Sweden.
Biotechnol J. 2011 Sep;6(9):1115-29. doi: 10.1002/biot.201100129. Epub 2011 Jul 22.
Technologies for display of recombinant protein libraries are today essential tools in many research-intensive fields, such as in the drug discovery processes of biopharmaceutical development. Phage display is still the most widely used method, but alternative systems are available and are becoming increasingly popular. The most rapidly expanding of the alternative systems are the cell display-based technologies, offering innovative strategies for selection and characterization of affinity proteins. Most investigations have focused on eukaryotic yeast for display of protein libraries, but similar systems are also being developed using prokaryotic hosts. This review summarizes the field of bacterial surface display with a strong emphasis on library applications for generation of new affinity proteins. The main focus will be on the most recent progress of the work on primarily Escherichia coli, but also on studies using a recently developed system for display on Gram-positive Staphylococcus carnosus. In addition, general strategies for combinatorial protein engineering using cell display are discussed along with the latest developments of new methodologies with comparisons to mainly phage display technology.
在许多研究密集型领域，如生物制药开发的药物发现过程中，用于展示重组蛋白文库的技术是今天必不可少的工具。噬菌体展示仍然是最广泛使用的方法，但也有替代系统可用，并且越来越受欢迎。替代系统中发展最快的是基于细胞展示的技术，为亲和蛋白的选择和表征提供了创新策略。大多数研究都集中在真核酵母上展示蛋白文库，但也正在使用原核宿主开发类似的系统。本综述总结了细菌表面展示领域，重点介绍了用于生成新亲和蛋白的文库应用。主要重点将放在大肠杆菌上的最新进展上，但也将讨论使用最近开发的用于革兰氏阳性肉葡萄球菌展示的系统的研究。此外，还讨论了使用细胞展示进行组合蛋白工程的一般策略，以及与主要噬菌体展示技术的比较的最新新方法学的发展。
