Faculty of Medicine, Department of Medical Microbiology, Gaziantep University, Gaziantep, Turkey.
Faculty of Medicine, Department of Medical Microbiology, Sanko University, Gaziantep, Turkey.
Int J Hyperthermia. 2021;38(1):1002-1012. doi: 10.1080/02656736.2021.1943546.
Swarming motility is a virulence factor for and is a coordinated multicellular movement of bacteria. In this study, we investigated the inhibitory effect of hyperthermia on bacterial swarming motility and antimicrobial resistance.
Thirty-one isolates were included in the study. Seven inoculated agar plates were incubated inside incubators with increasing temperature levels: at 36 °C (control) and 40-45 °C. On the next day, inhibition of swarming was evaluated and minimum paralyzing temperature (MPT) values were determined. An antimicrobial susceptibility test (antibiogram) is performed by exposing bacteria to increasing concentrations of antibiotics, . Thus, we used the Kirby-Bauer disk diffusion test as a screening method to analyze the antibiogram profiles of the isolates at 36 °C and 42 °C. Finally, a time-kill assay was performed to analyze the killing effect of hyperthermia (42 °C) on planktonic bacteria, in combination with the antibiotic meropenem at the first and third hours. A Wilcoxon signed-rank test was used to compare the killing effects of meropenem, hyperthermia and their combinations.
The median MPT value was determined as 44 °C. In the disk diffusion assay, susceptibility development was observed in 94% of isolates for at least one antibiotic. In the time-kill assay, we observed a significant killing effect of hyperthermia in combination with meropenem. Under the microscope, we observed the formation of spherical cells by the effect of heat.
We conclude that these findings might be useful when employing the hyperthermia method to treat infectious diseases caused by in the future.
群集运动是 的毒力因子，是细菌的一种协调的多细胞运动。在这项研究中，我们研究了高温对细菌群集运动和抗菌耐药性的抑制作用。
本研究纳入了 31 株 分离株。将 7 个接种琼脂平板放置在温度逐渐升高的培养箱中进行孵育：36°C（对照）和 40-45°C。次日，评估群集抑制作用并确定最小麻痹温度（MPT）值。通过将细菌暴露于逐渐增加的抗生素浓度来进行抗菌药物敏感性测试（药敏试验），即 。因此，我们使用 Kirby-Bauer 圆盘扩散试验作为筛选方法，分析在 36°C 和 42°C 下分离株的药敏谱。最后，进行时间杀伤试验以分析高温（42°C）对浮游菌的杀伤作用，并与抗生素美罗培南联合分析在第 1 小时和第 3 小时的杀伤效果。使用 Wilcoxon 符号秩检验比较美罗培南、高温及其组合的杀伤效果。
中位 MPT 值为 44°C。在圆盘扩散试验中，94%的分离株对至少一种抗生素表现出敏感性增加。在时间杀伤试验中，我们观察到高温与美罗培南联合使用具有显著的杀菌效果。在显微镜下，我们观察到由于热的影响形成了球形细胞。
我们的结论是，这些发现可能对未来使用高温方法治疗 引起的感染性疾病有用。
