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氟喹诺酮类药物和抗氧化剂对奇异变形杆菌生物膜形成的影响。

The effect of fluoroquinolones and antioxidans on biofilm formation by Proteus mirabilis strains.

机构信息

Department of Microbiology, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.

Department of Pharmaceutical Botany and Pharmacognosy, Nicolaus Copernicus University in Toruń, L. Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz, Poland.

出版信息

Ann Clin Microbiol Antimicrob. 2022 Jun 2;21(1):22. doi: 10.1186/s12941-022-00515-5.

DOI:10.1186/s12941-022-00515-5
PMID:35655208
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9161520/
Abstract

BACKGROUND

Fluoroquinolones are a group of antibiotics used in urinary tract infections. Unfortunately, resistance to this group of drugs is currently growing. The combined action of fluoroquinolones and other antibacterial and anti-biofilm substances may extend the use of this therapeutic option by clinicians. The aim of the study was to determine the effect of selected fluoroquinolones and therapeutic concentrations of ascorbic acid and rutoside on biofilm formation by Proteus mirabilis.

MATERIALS AND METHODS

The study included 15 strains of P. mirabilis isolated from urinary tract infections in patients of the University Hospital No. 1 dr A. Jurasz in Bydgoszcz (Poland). The metabolic activity of the biofilm treated with 0.4 mg/ml ascorbic acid, 0.02 µg/ml rutoside and chemotherapeutic agents (ciprofloxacin, norfloxacin) in the concentration range of 0.125-4.0 MIC (minimum inhibitory concentration) was assessed spectrophotometrically.

RESULTS

Both ciprofloxacin and norfloxacin inhibited biofilm formation by the tested strains. The biofilm reduction rate was correlated with the increasing concentration of antibiotic used. No synergism in fluoroquinolones with ascorbic acid, rutoside or both was found. The ascorbic acid and rutoside combination, however, significantly decreased biofilm production.

CONCLUSIONS

Our research proves a beneficial impact of ascorbic acid with rutoside supplementation on biofilm of P. mirabilis strains causing urinary tract infections.

摘要

背景

氟喹诺酮类药物是一组用于尿路感染的抗生素。不幸的是，目前该药物群体的耐药性正在上升。氟喹诺酮类药物与其他抗菌和抗生物膜物质联合作用，可能会延长临床医生对这一治疗选择的应用。本研究的目的是确定选定的氟喹诺酮类药物和治疗浓度的抗坏血酸和芦丁对奇异变形杆菌生物膜形成的影响。

材料和方法

本研究包括从波兰比得哥什 1 号 AJurasz 大学医院尿路感染患者中分离出的 15 株奇异变形杆菌。用 0.4mg/ml 抗坏血酸、0.02μg/ml 芦丁和化学治疗剂（环丙沙星、诺氟沙星）处理生物膜的代谢活性，评估范围为 0.125-4.0MIC（最小抑菌浓度）。

结果

环丙沙星和诺氟沙星均抑制了试验菌株的生物膜形成。生物膜减少率与所用抗生素浓度的增加呈正相关。未发现氟喹诺酮类药物与抗坏血酸、芦丁或两者之间有协同作用。然而，抗坏血酸和芦丁联合使用显著降低了生物膜的产生。

结论

我们的研究证明了抗坏血酸与芦丁联合补充对引起尿路感染的奇异变形杆菌生物膜的有益影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/ba830d85983c/12941_2022_515_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/1318193d695f/12941_2022_515_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/faf2317a8de7/12941_2022_515_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/cc2d40ae7b78/12941_2022_515_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/bfe49727e910/12941_2022_515_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/ba830d85983c/12941_2022_515_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/1318193d695f/12941_2022_515_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/faf2317a8de7/12941_2022_515_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/cc2d40ae7b78/12941_2022_515_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/bfe49727e910/12941_2022_515_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f35/9161520/ba830d85983c/12941_2022_515_Fig5_HTML.jpg

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