Department of Histology and Embryology, School of Medicine, Istanbul Medipol University, Istanbul, Turkey.
Research Institute for Health Sciences and Technologies (SABITA), İstanbul, Medipol University, Istanbul, Turkey.
Asian Pac J Cancer Prev. 2021 Jun 1;22(6):1745-1751. doi: 10.31557/APJCP.2021.22.6.1745.
Cell culture studies are an indispensable tools used to understand basic physiological, biophysical and biomolecular mechanisms. Although traditional two-dimensional (2D) cell culture models are more preferred in experimental studies, three-dimensional (3D) cell culture models, attract more attention due to several advantages including mimicking tumor physiology, biochemistry and biomechanics. We aimed to investigate the effects of Gallic Acid, an antimutagenic, antioxidant and anticarcinogenic agent, on both 2D and 3D cultured endometrial cancer cells for the first time.
IC50 values were determined in 2D and 3D cultured endometrial cancer cells exposed to different doses of GA. In the 2D culture model exposed to GA, Caspase 3 expression levels were analyzed. In addition, the effect of GA on the migration of 2D cultured endometrium cancer cells was investigated.
IC50 value in the 3D model was found significantly higher than the 2D model. In 2D culture model, Caspase 3 expression and apoptosis was increased significantly in cells of GA exposed group compared to the control group. GA did not have a significant effect on the migration profile of cells.
Gallic Acid is shown to induce apoptosis in Ishikawa cells via Caspase 3 activation. We demonstrated a significantly higher IC50 level in 3D model which provide evidence to prefer 3D models in drug-test trials. The data obtained in the current study will provide a basis for further experiments to analyze the effects of GA on endometrial cancer and to develop strategies for clinical treatment.
细胞培养研究是理解基本生理、生物物理和生物分子机制的不可或缺的工具。虽然传统的二维（2D）细胞培养模型在实验研究中更受欢迎，但由于模拟肿瘤生理学、生物化学和生物力学等几个优点，三维（3D）细胞培养模型越来越受到关注。我们旨在首次研究没食子酸（一种抗诱变、抗氧化和抗癌剂）对 2D 和 3D 培养的子宫内膜癌细胞的影响。
在暴露于不同剂量 GA 的 2D 和 3D 培养的子宫内膜癌细胞中确定 IC50 值。在暴露于 GA 的 2D 培养模型中，分析 Caspase 3 表达水平。此外，还研究了 GA 对 2D 培养的子宫内膜癌细胞迁移的影响。
发现 3D 模型中的 IC50 值明显高于 2D 模型。在 2D 培养模型中，与对照组相比，GA 暴露组细胞中的 Caspase 3 表达和细胞凋亡显著增加。GA 对细胞迁移谱没有显著影响。
没食子酸通过 Caspase 3 激活诱导 Ishikawa 细胞凋亡。我们证明了 3D 模型中的 IC50 水平明显更高，这为药物试验中优先选择 3D 模型提供了证据。本研究获得的数据将为进一步分析 GA 对子宫内膜癌的影响以及为临床治疗开发策略提供基础。
