癌症研究中的三维培养系统：聚焦肿瘤球模型。
Three-dimensional culture systems in cancer research: Focus on tumor spheroid model.
作者信息
Nath Sritama, Devi Gayathri R
机构信息
Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, Durham, NC 27710, United States.
Division of Surgical Sciences, Department of Surgery, Duke University School of Medicine, Durham, NC 27710, United States; Duke Cancer Institute, Women's Cancer Program, Duke University School of Medicine, Durham, NC 27710, United States.
出版信息
Pharmacol Ther. 2016 Jul;163:94-108. doi: 10.1016/j.pharmthera.2016.03.013. Epub 2016 Apr 8.
Abstract
Cancer cells propagated in three-dimensional (3D) culture systems exhibit physiologically relevant cell-cell and cell-matrix interactions, gene expression and signaling pathway profiles, heterogeneity and structural complexity that reflect in vivo tumors. In recent years, development of various 3D models has improved the study of host-tumor interaction and use of high-throughput screening platforms for anti-cancer drug discovery and development. This review attempts to summarize the various 3D culture systems, with an emphasis on the most well characterized and widely applied model - multicellular tumor spheroids. This review also highlights the various techniques to generate tumor spheroids, methods to characterize them, and its applicability in cancer research.
摘要
在三维（3D）培养系统中增殖的癌细胞表现出与生理相关的细胞-细胞和细胞-基质相互作用、基因表达和信号通路谱、异质性和结构复杂性，这些都反映了体内肿瘤的特征。近年来，各种3D模型的发展改善了宿主-肿瘤相互作用的研究以及用于抗癌药物发现和开发的高通量筛选平台的应用。本综述试图总结各种3D培养系统，重点介绍特征最明确、应用最广泛的模型——多细胞肿瘤球体。本综述还强调了生成肿瘤球体的各种技术、表征它们的方法及其在癌症研究中的适用性。
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