人唾液蛋白谱作为阿萨姆邦 Tamol 咀嚼者人群中口腔鳞状细胞癌的预测和预后工具。
Protein Profile of Human Saliva as a Predictive and Prognostic Tool for OSCC in Tamol Chewer's Population in Assam.
机构信息
Department of Pathology , Assam Downtown University, India.
Assam Down Town University Panikhaiti, Assam, India.
出版信息
Asian Pac J Cancer Prev. 2021 Jun 1;22(6):1837-1841. doi: 10.31557/APJCP.2021.22.6.1837.
OBJECTIVE
To identify potential proteomic salivary biomarker in tamol chewers and comparing it to healthy and Oral squamous cell carcinoma cases.
METHODS
A total of fifty unstimulated saliva samples were collected from the healthy volunteers, tamol chewers (without tobacco), and OSCC patients referred to North-East cancer Hospital, Jorabat, Assam, India. The 2-D gel analysis and western blotting were performed to analyze protein profiling.
RESULTS
The identified proteins were serum albumin, HSP (Heat shock protein) 27, gamma actin, SCC (Squamous cell carcinoma) 1, and Annexin A4. All the proteins were associated with OSCC development when their values were compared with those of normal healthy subjects. HSP27 was subjected to further validation using western blotting methods. An increase of 18.39% (Serum Albumin), 15.04% (gamma actin), 14.01% (SSC 1), and 20.22% (ANX4) were observed in Tamol chewers when compared with healthy control subjects.
CONCLUSION
Our results revealed that the identified salivary proteins have a positive association with OSCC development. Profiling of these saliva proteomes especially HSP (Heat shock protein) 27 as a potential biomarker for OSCC detection in the high-risk population is recommended.
目的
鉴定探美吸烟咀嚼者潜在的唾液蛋白质组生物标志物，并将其与健康者和口腔鳞状细胞癌（OSCC）病例进行比较。
方法
从印度阿萨姆邦约拉巴特东北癌症医院的健康志愿者、探美咀嚼者（无烟草）和 OSCC 患者中收集了 50 份未刺激的唾液样本。通过二维凝胶分析和 Western blot 进行蛋白质谱分析。
结果
鉴定出的蛋白质为血清白蛋白、热休克蛋白（HSP）27、γ肌动蛋白、SCC（鳞状细胞癌）1 和膜联蛋白 A4。当将这些蛋白质的数值与正常健康受试者进行比较时，所有蛋白质都与 OSCC 的发展有关。使用 Western blot 方法进一步验证了 HSP27。与健康对照组相比，探美咀嚼者的血清白蛋白增加了 18.39%，γ肌动蛋白增加了 15.04%，SCC1 增加了 14.01%，膜联蛋白 A4 增加了 20.22%。
结论
我们的结果表明，鉴定出的唾液蛋白质与 OSCC 的发展呈正相关。建议对这些唾液蛋白质组进行分析，特别是 HSP（热休克蛋白）27 作为高危人群中 OSCC 检测的潜在生物标志物。
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