Key Laboratory of Structure and Functional Regulation of Hybrid Materials, Ministry of Education, Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China.
Institute of Immunology and the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230027, China.
Anal Chem. 2021 Jul 13;93(27):9304-9308. doi: 10.1021/acs.analchem.1c02145. Epub 2021 Jun 28.
Early diagnosis is crucial to the treatment of cancer. Cathepsin B (CTB) plays an important role in numerous cancers, which is a promising biomarker for early diagnosis of cancer. It is necessary to exploit new probes for visualization of CTB . Fluorescent/photoacoustic (FL/PA) imaging is a powerful tool for study which possesses both excellent sensitivity and spatial resolution. To our knowledge, there has been no FL/PA probe to image CTB or . Therefore, we developed two CTB-activated FL/PA probes and , which could successfully monitor CTB activity . Both two probes had excellent sensitivity and selectivity . Cell imaging showed that or could image endogenous CTB in lysosome with 6.8-fold or 5.1-fold enhancement of the FL signal and 5.8-fold or 3.4-fold enhancement of the PA signal compared to their inhibitor contrast groups. Tumor imaging further confirmed the good applicability of these two probes to monitor CTB activity with high sensitivity and spatial resolution. Moreover, the property of is superior to due to the higher catalytic efficiency of CTB toward than . We envision that our FL/PA probe will be suitable for clinical early diagnosis of CTB-related cancer in the near future.
早期诊断对于癌症的治疗至关重要。组织蛋白酶 B（CTB）在许多癌症中发挥着重要作用，是癌症早期诊断的有前途的生物标志物。有必要开发新的探针来可视化 CTB。荧光/光声（FL/PA）成像是一种强大的研究工具，具有出色的灵敏度和空间分辨率。据我们所知，目前还没有用于成像 CTB 或 的 FL/PA 探针。因此，我们开发了两种 CTB 激活的 FL/PA 探针和，它们可以成功监测 CTB 活性。这两种探针都具有出色的灵敏度和选择性。细胞成像表明，或在溶酶体中可以成像内源性 CTB，与抑制剂对照组相比，FL 信号增强了 6.8 倍或 5.1 倍，PA 信号增强了 5.8 倍或 3.4 倍。肿瘤成像进一步证实了这两种探针具有高灵敏度和空间分辨率监测 CTB 活性的良好适用性。此外，由于 CTB 对的催化效率高于，因此的性能优于。我们设想我们的 FL/PA 探针将在不久的将来适合用于临床早期诊断与 CTB 相关的癌症。
