Shi Yanhua, Wang Xianghui, Zhang Shifang, Yin Hao, Fan Huaju, Tang Yaohui, Yang Nana
School of Bioscience and Technology, Weifang Medical University, Weifang 261053, Shandong, China.
Medical Laboratory Animal Center, Weifang Medical University, Weifang 261053, Shandong, China.
Extracell Vesicles Circ Nucl Acids. 2023 Dec 7;4(4):684-697. doi: 10.20517/evcna.2023.49. eCollection 2023.
Cells have the capability to discharge extracellular vesicles (EVs) into a range of bodily fluids. Extracellular vesicles (EVs) encapsulate biological molecules such as proteins and nucleic acids, playing a role in facilitating cell-cell communication. They actively engage in a myriad of physiological and pathological processes. tracing of EVs in organisms significantly contributes to elucidating the biological mechanisms of EV-based therapy. The development of molecular imaging technology makes it possible to trace EVs . Experiments frequently employ a range of molecular imaging techniques, encompassing bioluminescence imaging, fluorescence imaging, magnetic resonance imaging, single photon emission computed tomography, positron emission tomography, photoacoustic imaging, and multimodal imaging. These methods have their own advantages and disadvantages. In this review, typical applications of tracing of EVs are reviewed.
细胞具有将细胞外囊泡（EVs）释放到一系列体液中的能力。细胞外囊泡（EVs）包裹着蛋白质和核酸等生物分子，在促进细胞间通讯中发挥作用。它们积极参与众多生理和病理过程。在生物体内追踪细胞外囊泡对阐明基于细胞外囊泡疗法的生物学机制有重要贡献。分子成像技术的发展使追踪细胞外囊泡成为可能。实验中经常采用一系列分子成像技术，包括生物发光成像、荧光成像、磁共振成像、单光子发射计算机断层扫描、正电子发射断层扫描、光声成像和多模态成像。这些方法各有优缺点。在本综述中，将对细胞外囊泡追踪的典型应用进行综述。
