The Center for Drug Research and Development, Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou 510006, China.
School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Shenzhen 518107, China.
Molecules. 2023 Jan 30;28(3):1329. doi: 10.3390/molecules28031329.
(), a Fabaceae family member, is widely used as an anti-inflammatory herbal medicine; however, its antibacterial and antidiabetic properties have not been extensively investigated. This study aimed to systematically analyze the antibacterial and antidiabetic components of by utilizing a combination of analytical methods. First, ten different polarity extracts were analyzed through ultra-performance liquid chromatography (UPLC), and their antibacterial and antidiabetic activities were evaluated. Then the spectrum-effect relationship between the biological activity and UPLC chromatograms was analyzed by partial least squares regression and gray relational analysis, followed by corresponding validation using isolated components. Finally, network pharmacology and molecular docking were implemented to predict the main antibacterial target components of and the enzyme inhibition active sites of α-amylase and α-glucosidase. P15, P16, and P20 were found to be the antibacterial and antidiabetic active components. The inhibitory effect of 7-O-galloyltricetiflavan (P15) on six bacterial species may be mediated through the lipid and atherosclerosis pathway, prostate cancer, adherens junctions, and targets such as SRC, MAPK1, and AKT1. The molecular docking results revealed that 7-O-galloyltricetiflavan and 7,4'-di-O-galloyltricetiflavan (P16/P20) can bind to α-amylase and α-glucosidase pockets with binding energies lower than -6 kcal/mol. Our study provides guidance for the development of antibacterial and antidiabetic products based on and can be used as a reference for the evaluation of bioactivity of other herbs.
（），豆科植物家族的一员，被广泛用作抗炎草药；然而，其抗菌和降血糖特性尚未得到广泛研究。本研究旨在利用分析方法的组合，系统分析 的抗菌和降血糖成分。首先，通过超高效液相色谱（UPLC）分析了十种不同极性的提取物，并评估了它们的抗菌和降血糖活性。然后，通过偏最小二乘回归和灰色关联分析对生物活性与 UPLC 图谱之间的谱效关系进行了分析，随后使用分离的成分进行了相应的验证。最后，通过网络药理学和分子对接预测了 的主要抗菌目标成分和α-淀粉酶和α-葡萄糖苷酶的酶抑制活性位点。P15、P16 和 P20 被发现是具有抗菌和降血糖活性的成分。7-O-没食子酰三咖啡酰奎宁酸（P15）对六种细菌的抑制作用可能是通过脂质和动脉粥样硬化途径、前列腺癌、黏着连接以及 SRC、MAPK1 和 AKT1 等靶点介导的。分子对接结果表明，7-O-没食子酰三咖啡酰奎宁酸和 7,4'-二-O-没食子酰三咖啡酰奎宁酸（P16/P20）可以与α-淀粉酶和α-葡萄糖苷酶口袋结合，结合能低于-6 kcal/mol。本研究为基于 的抗菌和降血糖产品的开发提供了指导，也可以作为评价其他草药生物活性的参考。
