Furchgott Robert F
Department of Pharmacology, Box 29, SUNY Health Science Center at Brooklyn, 450 Clarkson Avenue, Brooklyn, NY 11203 (USA), Fax: (+1) 718-270-2241.
Angew Chem Int Ed Engl. 1999 Jul 12;38(13-14):1870-1880. doi: 10.1002/(SICI)1521-3773(19990712)38:13/14<1870::AID-ANIE1870>3.0.CO;2-8.
The presence of air bubbles, and thus the unintentional removal of endothelial cells, may have prevented the discovery of endothelium-dependent vasodilation at an earlier date. In investigations with perfused rabbit central ear artery the infusion of acetylcholine, a known potent prejunctional inhibitor of adrenergic neurotransmission, did not inhibit vasoconstriction produced by infused norepinephrine. It was later determined that air bubbles in the perfusion line had mechanically removed the endothelial cells, and as a result no release of endothelium-derived relaxing factor (EDRF) could take place.
气泡的存在，以及由此导致的内皮细胞意外去除，可能阻碍了内皮依赖性血管舒张的早期发现。在对灌注兔中耳动脉的研究中，注入已知的强效肾上腺素能神经传递前体抑制剂乙酰胆碱，并未抑制注入去甲肾上腺素所产生的血管收缩。后来发现，灌注管路中的气泡机械性地去除了内皮细胞，因此无法释放内皮衍生舒张因子（EDRF）。
