Division of Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, Gujarat, 364002, India.
Academy of Scientific and Innovative Research (AcSIR), CSIR, Ghaziabad, India.
Sci Rep. 2021 Jun 28;11(1):13435. doi: 10.1038/s41598-021-92424-4.
Stenotrophomonas maltophilia has plant growth-promoting potential, and interaction with Arachis hypogaea changes host-plant physiology, biochemistry, and metabolomics, which provides tolerance under the N starvation conditions. About 226 suppression subtractive hybridization clones were obtained from plant-microbe interaction, of which, about 62% of gene sequences were uncharacterized, whereas 23% of sequences were involved in photosynthesis. An uncharacterized SSH clone, SM409 (full-length sequence showed resemblance with Cytb6), showed about 4-fold upregulation during the interaction was transformed to tobacco for functional validation. Overexpression of the AhCytb6 gene enhanced the seed germination efficiency and plant growth under N deficit and salt stress conditions compared to wild-type and vector control plants. Results confirmed that transgenic lines maintained high photosynthesis and protected plants from reactive oxygen species buildup during stress conditions. Microarray-based whole-transcript expression of host plants showed that out of 272,410 genes, 8704 and 24,409 genes were significantly (p < 0.05) differentially expressed (> 2 up or down-regulated) under N starvation and salt stress conditions, respectively. The differentially expressed genes belonged to different regulatory pathways. Overall, results suggested that overexpression of AhCytb6 regulates the expression of various genes to enhance plant growth under N deficit and abiotic stress conditions by modulating plant physiology.
寡养单胞菌具有促进植物生长的潜力，与花生的相互作用改变了宿主植物的生理学、生物化学和代谢组学，从而提供了在氮饥饿条件下的耐受能力。从植物-微生物相互作用中获得了约 226 个抑制性消减杂交克隆，其中约 62%的基因序列尚未确定，而 23%的序列与光合作用有关。一个未被描述的 SSH 克隆 SM409（全长序列与 Cytb6 相似）在相互作用过程中表达上调了约 4 倍，被转化为烟草进行功能验证。与野生型和载体对照植物相比，AhCytb6 基因的过表达提高了种子发芽效率和氮缺乏及盐胁迫条件下的植物生长。结果证实，与胁迫条件下的野生型和载体对照植物相比，转基因株系保持了较高的光合作用，并能防止植物体内活性氧的积累。基于微阵列的宿主植物全转录表达显示，在 272410 个基因中，有 8704 个和 24409 个基因在氮饥饿和盐胁迫条件下分别显著（p<0.05）差异表达（上调或下调>2 倍）。差异表达的基因属于不同的调控途径。总的来说，结果表明，AhCytb6 的过表达通过调节植物生理学，调控各种基因的表达，从而在氮亏缺和非生物胁迫条件下增强植物生长。
