Regenmedix Consulting LLC, Houston, TX, 7030, USA.
Lillehei Heart Institute, Univeristy of Minnesota, USA.
Physiol Rep. 2021 Jun;9(12):e14817. doi: 10.14814/phy2.14817.
To expand the application of perfusion decellularization beyond isolated single organs, we used the native vasculature of adult and neonatal rats to systemically decellularize the organs of a whole animal in situ. Acellular scaffolds were generated from kidney, liver, lower limb, heart-lung system, and a whole animal body, demonstrating that perfusion decellularization technology is applicable to any perfusable tissue, independent of age. Biochemical and histological analyses demonstrated that organs and organ systems (heart-lung pair and lower limb) were successfully decellularized, retaining their extracellular matrix (ECM) structure and organ-specific composition, as evidenced by differences in organ-specific scaffold stiffness. Altogether, we demonstrated that organs, organ systems and whole animal bodies can be perfusion decellularized while retaining ECM components and biomechanics.
为了将灌注去细胞化的应用扩展到单个器官之外，我们使用成年和新生大鼠的天然脉管系统，在原位对整个动物的器官进行系统性去细胞化。从肾脏、肝脏、下肢、心肺系统和整个动物体中生成了无细胞支架，表明灌注去细胞化技术适用于任何可灌注的组织，与年龄无关。生化和组织学分析表明，器官和器官系统（心肺对和下肢）成功地进行了去细胞化，保留了它们的细胞外基质（ECM）结构和器官特异性组成，这一点可以通过器官特异性支架硬度的差异来证明。总的来说，我们证明了器官、器官系统和整个动物体可以在保留 ECM 成分和生物力学的情况下进行灌注去细胞化。
