School of Food and Biological Engineering, Hefei University of Technology, Hefei, Anhui 230009, China.
Department of Traditional Chinese Medicine, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui 230001, China.
Anal Chem. 2021 Jul 13;93(27):9299-9303. doi: 10.1021/acs.analchem.1c01537. Epub 2021 Jun 29.
The development of precise medicine requires diagnostic probes to simultaneously satisfy an excellent detection limit and a wide linear analysis range because of enormous individual-discrepancy of disease biomarker concentrations, yet it remains challenging. Herein, an upconverison nanoprobe with a luminescence ratio flexibly tailored was designed for ultrasensitive monitoring exhaled nitric oxide to indicate the clinical course of asthma. Two independent emissions from the same nanoprobe can be discretionarily modulated to vary their intensity ratios for adapting different analysis requirements. Delightfully, this novel nanoprobe demonstrated a 100-fold lower detection limit compared with the traditional ratiometric fluorescence manner and a more broad linear detection range from the subpart per billion (ppb) level to hundreds of ppb. This ratio-adjustable fluorescence detection strategy holds great potential for miscellaneous disease diagnosis applications.
精准医学的发展需要诊断探针同时满足优异的检测限和宽的线性分析范围，因为疾病生物标志物浓度存在巨大的个体差异，但这仍然具有挑战性。在此，设计了一种上转换纳米探针，其荧光比率可灵活调节，用于超灵敏监测呼出气一氧化氮，以指示哮喘的临床过程。同一纳米探针的两个独立发射可以任意调节，以改变它们的强度比，以适应不同的分析要求。令人高兴的是，与传统的比率荧光方式相比，这种新型纳米探针的检测限低了 100 倍，从亚部分每十亿（ppb）到几百 ppb 的线性检测范围也更宽。这种可调节比率的荧光检测策略在各种疾病诊断应用中具有很大的潜力。
