Institut de Recherche en Science de la Santé - Direction régionale du Centre-Ouest/Unité de Recherche Clinique de Nanoro, Nanoro, Burkina Faso.
Department of Medical Microbiology, Experimental Parasitology Unit, Amsterdam University Medical Centers, Academic Medical Center at the University of Amsterdam, Amsterdam, The Netherlands.
Trop Med Int Health. 2021 Oct;26(10):1220-1230. doi: 10.1111/tmi.13644. Epub 2021 Jul 21.
Antibiotics efficacy is severely threatened due to emerging resistance worldwide, but there is a paucity of antibiotics efficacy data for the West African region in general. Therefore, this study aimed to determine the antibiotic susceptibility profile of bacterial isolated from febrile children under 5 years of age in Nanoro (Burkina Faso).
Blood, stool and urine samples were collected from 1099 febrile children attending peripheral health facilities and the referral hospital in Nanoro Health district. Bacterial isolates from these samples were assessed for their susceptibility against commonly used antibiotics by Kirby-Bauer method.
In total, 141 bacterial isolates were recovered from 127 febrile children of which 65 from blood, 65 from stool and 11 from urine. Salmonella isolates were most frequently isolated and found to be highly resistant to ampicillin (70%; 56/80) and trimethoprim-sulphamethoxazole (65%; 52/80). Escherichia coli isolates showed a high resistance rate to trimethoprim-sulphamethoxazole (100%), ampicillin (100%), ciprofloxacin (71.4%; 10/14), amoxicillin-clavulanate (64.3%; 9/14), ceftriaxone (64.3%; 9/14) and gentamycin (50%; 7/14). Moreover, half of the E. coli isolates produced ß-lactamase suggesting multi-drug resistance against β-lactam as well as non-β-lactam antibiotics. Multi-drug resistance was observed in 54.6% (59/108) of the isolates, mainly Gram-negative bacteria.
This study showed high resistance rates to common antibiotics used to treat bacterial infections in Nanoro. The work prompts the need to expand antibiotic resistance surveillance studies in Burkina Faso.
由于世界各地不断出现耐药性，抗生素的疗效受到严重威胁，但一般来说，西非地区的抗生素疗效数据很少。因此，本研究旨在确定纳诺罗（布基纳法索）发热 5 岁以下儿童血液、粪便和尿液样本中分离的细菌对抗生素的敏感性。
从纳诺罗卫生区的周边卫生机构和转诊医院采集了 1099 名发热儿童的血液、粪便和尿液样本。采用 Kirby-Bauer 法评估这些样本中的细菌分离物对常用抗生素的敏感性。
共从 127 名发热儿童的 141 株细菌分离物中回收，其中 65 株来自血液，65 株来自粪便，11 株来自尿液。沙门氏菌分离物最常被分离出来，对氨苄西林（70%；56/80）和磺胺甲恶唑-甲氧苄啶（65%；52/80）高度耐药。大肠杆菌分离物对磺胺甲恶唑-甲氧苄啶（100%）、氨苄西林（100%）、环丙沙星（71.4%；10/14）、阿莫西林-克拉维酸（64.3%；9/14）、头孢曲松（64.3%；9/14）和庆大霉素（50%；7/14）的耐药率很高。此外，一半的大肠杆菌分离物产生β-内酰胺酶，表明对β-内酰胺类和非β-内酰胺类抗生素具有多药耐药性。108 株分离物中有 54.6%（59/108）表现出多药耐药性，主要为革兰氏阴性菌。
本研究表明，纳诺罗常用抗生素治疗细菌感染的耐药率较高。这项工作促使我们有必要在布基纳法索扩大抗生素耐药性监测研究。
