Mulu Berihun Mossie, Belete Mequanint Addisu, Demlie Tiliksew Bialfew, Tassew Habtamu, Sisay Tessema Tesfaye
Department of Veterinary Science, College of Agriculture and Environmental Sciences, Bahir Dar University, Bahir Dar 79, Ethiopia.
Department of Veterinary Laboratory Technology, College of Agriculture and Natural Resource, Debre Markos University, Debre Markos 269, Ethiopia.
Trop Med Infect Dis. 2024 Mar 21;9(3):65. doi: 10.3390/tropicalmed9030065.
Diarrheagenic (DEC) are the leading cause of infectious diarrhea and pose a significant global, regional, and national burden of disease. This study aimed to investigate the prevalence of six DEC pathotypes in children with diarrhea and determine their antibiotic resistance patterns. Samples from 107 diarrheagenic children were collected and processed for (). Single-plex PCR was used to detect target virulence genes as well as characterize and categorize DEC pathotypes. Antibiotic resistance patterns were determined by the Kirby-Bauer disk diffusion method. was detected in 79 diarrheal stool samples, accounting for 73.8% of the samples collected. Additionally, 49.4% (39 out of 79) of the isolates harbored various typical virulence factors. Results revealed six pathotypes of virulence: enterotoxigenic (ETEC) (53.8%), enteropathogenic (EPEC) (12.8%), enteroaggregative (EAEC) (10.3%), Heteropathotypes (7.8%), Shiga toxin-producing (STEC), and enterohemorrhagic (EHEC) (7.7% each). The isolates exhibited high antibiotic resistance against trimethoprim/sulfamethoxazole (82.1%), amoxicillin (79.5%), ampicillin (74.4%), gentamicin (69.2%), and streptomycin (64.1%). An overall occurrence of 84.6% of multiple-drug resistance was observed in the isolates, with resistance ranging from three to four antibiotic classes. Our findings revealed a high level of pathogenic that were highly resistant to multiple categories of antibiotics among children in the Awi zone. These findings highlight the potential role of pathogenic in childhood diarrhea in tropical low-resource settings and underscore the need for continued research on the characteristics of pathogenic and antibiotic-resistant strains.
致泻性大肠埃希菌（DEC）是感染性腹泻的主要病因，在全球、区域和国家层面均造成了重大的疾病负担。本研究旨在调查腹泻儿童中六种DEC致病型的流行情况，并确定其抗生素耐药模式。收集了107例腹泻儿童的样本并进行处理（）。采用单重PCR检测目标毒力基因，并对DEC致病型进行鉴定和分类。通过 Kirby-Bauer 纸片扩散法确定抗生素耐药模式。在79份腹泻粪便样本中检测到（），占所采集样本的73.8%。此外，49.4%（79份中的39份）的分离株携带各种典型毒力因子。结果显示出六种毒力致病型：产肠毒素性大肠埃希菌（ETEC）（53.8%）、肠致病性大肠埃希菌（EPEC）（12.8%）、肠集聚性大肠埃希菌（EAEC）（10.3%）、异型致病型（7.8%）、产志贺毒素大肠埃希菌（STEC）以及肠出血性大肠埃希菌（EHEC）（各占7.7%）。这些分离株对甲氧苄啶/磺胺甲恶唑（82.1%）、阿莫西林（79.5%）、氨苄西林（74.4%）、庆大霉素（69.2%）和链霉素（64.1%）表现出高度耐药性。在所分离的菌株中观察到多重耐药的总体发生率为84.6%，耐药范围为三到四类抗生素。我们的研究结果显示，在阿维地区儿童中，致病大肠埃希菌水平较高，且对多类抗生素具有高度耐药性。这些发现凸显了致病大肠埃希菌在热带资源匮乏地区儿童腹泻中的潜在作用，并强调了持续研究致病菌株和抗生素耐药菌株特征的必要性。
