School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
University of Guam Marine Laboratory, UOG Station, Mangilao, Guam 96923, USA.
Chembiochem. 2021 Aug 17;22(16):2614-2618. doi: 10.1002/cbic.202100275. Epub 2021 Jul 8.
Proline-rich macrocyclic peptides (PRMPs) are natural products present in geographically and phylogenetically dispersed marine sponges. The large diversity and low abundance of PRMPs in sponge metabolomes precludes isolation and structure elucidation of each individual PRMP congener. Here, using standards developed via biomimetic enzymatic synthesis of PRMPs, a mass spectrometry-based workflow to sequence PRMPs was developed and validated to reveal that the diversity of PRMPs in marine sponges is much greater than that has been realized by natural product isolation-based strategies. Findings are placed in the context of diversity-oriented transamidative macrocyclization of peptide substrates in sponge holobionts.
富含脯氨酸的大环肽（PRMPs）是存在于地理上和系统发育上分散的海洋海绵中的天然产物。海绵代谢组中 PRMP 的多样性大且丰度低，这使得每个单独的 PRMP 同系物的分离和结构阐明变得困难。在这里，通过 PRMP 的仿生酶合成来制定标准，开发并验证了一种基于质谱的 PRMP 测序工作流程，结果表明海洋海绵中 PRMP 的多样性比基于天然产物分离的策略所认识到的要大得多。研究结果被置于海绵共生体中肽底物的多样性导向的转酰胺化大环化的背景下。
