基于ε-聚赖氨酸的磁性纳米球作为一种高效、可回收的耐热环状芽孢杆菌抗菌剂。
Epsilon-polylysine based magnetic nanospheres as an efficient and recyclable antibacterial agent for Alicyclobacillus acidoterrestris.
机构信息
College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling 712100, China.
College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling 712100, China; College of Food Science and Technology, Northwest University, Xi'an 710069, China.
出版信息
Food Chem. 2021 Dec 1;364:130382. doi: 10.1016/j.foodchem.2021.130382. Epub 2021 Jun 21.
In this work, polyacrylic acid modified ferroferric oxide (FeO-PAA) was synthesized via a one-step hydrothermal method. The magnetic nanospheres modified with carboxyl groups were combined with epsilon-polylysine (EPL) via an EDC/NHS coupling reaction to obtain FeO-PAA-EPL nanospheres. FeO-PAA-EPL was employed as an antibacterial agent against Alicyclobacillus acidoterrestris and characterized by XRD, FTIR, XPS, VSM, SEM and TEM techniques. Experimental results showed the minimum inhibition concentration (MIC) of FeO-PAA-EPL against A. acidoterrestris was 1.25 mg mL. Furthermore, A. acidoterrestris treated with FeO-PAA-EPL nanospheres obviously lysed. Morphological analysis of bacteria supported by SEM indicated that the cell membrane of A. acidoterrestris was damaged, revealing that FeO-PAA-EPL is an effective antibacterial agent. Additionally, the nanospheres with excellent magnetism can be simply separated from a reaction system via an external magnet. The construction of magnetic nanospheres with satisfactory antibacterial activity provides an effective and new method to control A. acidoterrestris.
在这项工作中，通过一步水热法合成了聚丙烯酸修饰的四氧化三铁（FeO-PAA）。通过 EDC/NHS 偶联反应将带有羧基的磁性纳米球与 ε-聚赖氨酸（EPL）结合，得到 FeO-PAA-EPL 纳米球。FeO-PAA-EPL 被用作抗嗜酸耐热菌（Alicyclobacillus acidoterrestris）的抗菌剂，并通过 XRD、FTIR、XPS、VSM、SEM 和 TEM 技术进行了表征。实验结果表明，FeO-PAA-EPL 对 A. acidoterrestris 的最小抑菌浓度（MIC）为 1.25 mg mL。此外，用 FeO-PAA-EPL 纳米球处理的 A. acidoterrestris 明显裂解。SEM 支持的细菌形态分析表明，嗜酸耐热菌的细胞膜受到了损伤，表明 FeO-PAA-EPL 是一种有效的抗菌剂。此外，具有优异磁性的纳米球可以通过外部磁铁从反应体系中简单分离。具有令人满意抗菌活性的磁性纳米球的构建为控制嗜酸耐热菌提供了一种有效且新颖的方法。
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