Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, School of Public Health, Guangxi Medical University, Nanning, China,
Guangxi Key Laboratory of AIDS Prevention and Treatment & Guangxi Universities Key Laboratory of Prevention and Control of Highly Prevalent Disease, School of Public Health, Guangxi Medical University, Nanning, China.
Intervirology. 2022;65(1):58-66. doi: 10.1159/000517052. Epub 2021 Jun 29.
New kinds of HIV-1 circulating recombinant forms (CRFs) and unique recombinant forms (URFs) earn a great prevalence in China nowadays. In this study, we identified 2 similar URFs (2016GXNNIDU037 and 2019QZLSIDU253) both isolated from intravenous drug users (IDUs) in Guangxi, China. Phylogenetic analysis of the near full-length genome (NFLG) revealed 2 URFs both clustered with CRF01_AE but setting up a monophyletic branch, supporting a high bootstrap value. Bootscan analysis and subregional recombinant analysis found that the NFLG of 2016GXNNIDU037 and 2019QZLSIDU253 were both composed of CRF01_AE and CRF07_BC, with 3 CRF07_BC mosaic segments inserted into CRF01_AE backbones. The CRF01_AE segments of the 2 URFs clustered with a previously reported cluster 2 lineage of CRF01_AE. The 5 recombinant breakpoints of the 2 URFs were quite similar. Distinct from CRF01_AE/CRF07_BC URFs reported before, 2016GXNNIDU037 and 2019QZLSIDU253 are new evidence of a high genetic variety of HIV-1 in Guangxi, which may pose new challenges to HIV-1 prevention and molecular epidemiological surveillance in China.
目前，中国新出现了多种 HIV-1 循环重组形式（CRFs）和独特重组形式（URFs）。本研究鉴定了两株相似的 URFs（2016GXNNIDU037 和 2019QZLSIDU253），均分离自中国广西的静脉吸毒者（IDUs）。对接近全长基因组（NFLG）的系统发育分析表明，这两株 URF 均与 CRF01_AE 聚类，但形成了一个单系分支，支持高 bootstrap 值。Bootscan 分析和亚区重组分析发现，2016GXNNIDU037 和 2019QZLSIDU253 的 NFLG 均由 CRF01_AE 和 CRF07_BC 组成，有 3 个 CRF07_BC 嵌合片段插入 CRF01_AE 骨架中。这两株 URF 的 CRF01_AE 片段聚类为先前报道的 CRF01_AE 谱系 2 簇。这两株 URF 的 5 个重组断点非常相似。与之前报道的 CRF01_AE/CRF07_BC URF 不同，2016GXNNIDU037 和 2019QZLSIDU253 为广西 HIV-1 遗传多样性高提供了新证据，这可能给中国的 HIV-1 预防和分子流行病学监测带来新挑战。
