Department of Pediatric Surgery, Faculty of Medicine, University of Firat, Elazig, Turkey.
Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, Elazig, Turkey.
Parasitology. 2021 Oct;148(12):1482-1489. doi: 10.1017/S0031182021001098. Epub 2021 Jun 30.
Cystic echinococcosis (CE) is a neglected zoonotic tropical disease caused by Echinococcus granulosus sensu lato. The aim of this study was to investigate the genetic variation of hydatid cyst isolates obtained from surgically confirmed paediatric cases originating from two different regions in eastern Turkey. Seventeen paediatric cases aged between 6 and 16 were operated by open surgery, and the germinal layers of their cysts were obtained for further molecular analyses. After genomic DNA isolation, 875 bp mt-CO1 gene fragments were amplified in all samples by PCR. Then, the unidirectional sequence analyses of the PCR products were carried out. According to the BLAST analyses of 17 sequences, 16 of these sequences were matched with E. granulosus sensu stricto, while one sequence was identified as E. canadensis (G6/G7) for the first time in paediatric cases in Turkey. High haplotype diversity and low nucleotide diversity were observed in the E. granulosus s.s. sequences.
泡型包虫病（CE）是一种被忽视的热带动物源性人畜共患疾病，由细粒棘球绦虫亚种引起。本研究旨在调查从土耳其东部两个不同地区经手术证实的儿科病例中获得的包虫囊肿分离株的遗传变异。17 名年龄在 6 至 16 岁之间的儿科患者接受了开放式手术，从他们的囊肿的生发层获得了进一步的分子分析材料。在基因组 DNA 分离后，通过 PCR 扩增了所有样本中的 875 bp mt-CO1 基因片段。然后，对 PCR 产物进行单向序列分析。根据 17 个序列的 BLAST 分析，其中 16 个序列与细粒棘球绦虫亚种相符，而一个序列首次被鉴定为在土耳其儿科病例中的棘球蚴（G6/G7）。在细粒棘球绦虫亚种序列中观察到高单倍型多样性和低核苷酸多样性。
