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T 细胞和 B 细胞经高内皮微静脉腔周窗孔的逐步迁移。

Stepwise transmigration of T- and B cells through a perivascular channel in high endothelial venules.

机构信息

Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea.

Center for Vascular Research, Institute for Basic Science, Daejeon, Republic of Korea.

出版信息

Life Sci Alliance. 2021 Jun 29;4(8). doi: 10.26508/lsa.202101086. Print 2021 Aug.

DOI:10.26508/lsa.202101086
PMID:34187874
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9715433/
Abstract

High endothelial venules (HEVs) effectively recruit circulating lymphocytes from the blood to lymph nodes. HEVs have endothelial cells (ECs) and perivascular sheaths consisting of fibroblastic reticular cells (FRCs). Yet, post-luminal lymphocyte migration steps are not well elucidated. Herein, we performed intravital imaging to investigate post-luminal T- and B-cell migration in popliteal lymph node, consisting of trans-EC migration, crawling in the perivascular channel (a narrow space between ECs and FRCs) and trans-FRC migration. The post-luminal migration of T cells occurred in a PNAd-dependent manner. Remarkably, we found hot spots for the trans-EC and trans-FRC migration of T- and B cells. Interestingly, T- and B cells preferentially shared trans-FRC migration hot spots but not trans-EC migration hot spots. Furthermore, the trans-FRC T-cell migration was confined to fewer sites than trans-EC T-cell migration, and trans-FRC migration of T- and B cells preferentially occurred at FRCs covered by CD11c+ dendritic cells in HEVs. These results suggest that HEV ECs and FRCs with perivascular DCs delicately regulate T- and B-cell entry into peripheral lymph nodes.

摘要

高内皮小静脉 (HEVs) 可有效地将循环淋巴细胞从血液中招募到淋巴结中。HEVs 由内皮细胞 (ECs) 和由纤维母细胞网状细胞 (FRCs) 组成的血管周鞘组成。然而，后腔淋巴细胞迁移步骤尚未得到很好的阐明。在此，我们通过活体成像技术研究了位于腘淋巴结中的后腔 T 细胞和 B 细胞迁移，包括跨 EC 迁移、在血管周腔（ECs 和 FRCs 之间的狭窄空间）中爬行以及跨 FRC 迁移。T 细胞的后腔迁移是依赖于 PNAd 的。值得注意的是，我们发现了 T 细胞和 B 细胞跨 EC 和跨 FRC 迁移的热点。有趣的是，T 细胞和 B 细胞优先共享跨 FRC 迁移热点，而不是跨 EC 迁移热点。此外，与跨 EC T 细胞迁移相比，跨 FRC T 细胞迁移局限于更少的部位，并且 T 细胞和 B 细胞的跨 FRC 迁移优先发生在 HEV 中被 CD11c+树突状细胞覆盖的 FRC 上。这些结果表明，HEV 的 ECs 和 FRCs 与血管周 DC 一起精细地调节 T 细胞和 B 细胞进入外周淋巴结。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f9/9715433/95b77b904dc0/LSA-2021-01086_FigS12.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f9/9715433/efceeafb27ce/LSA-2021-01086_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9f9/9715433/09a6864c80ab/LSA-2021-01086_FigS1.jpg
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