Department of Aquaculture, Kerala University of Fisheries and Ocean Studies, Panangad, Kochi 682 506, India.
J Genet. 2021;100.
The freshwater leaf fish belongs to the family Pristolepididae, restricted to Pamba and Chalakudy rivers of Kerala, India. In the present study, we sequenced the complete mitogenome of and analysed its phylogeny in the order Anabantiformes. The 16622-bp long genome comprised of 13 protein-coding genes, two rRNA genes, 22 transfer RNAs (tRNAs) genes and had a noncoding control region. All the protein-coding genes, tRNA and rRNA were located on the heavy strand, except nad6 and eight tRNAs (glutamine, alanine, asparagine, cysteine, tyrosine, serine, glutamic acid and proline) transcribed from L strand. The genome exhibited an overlapping between atp8 and atp6 (2 bp), nad4 and nad4l (2 bp), tRNA and tRNA. (1 bp), tRNA and tRNA (1 bp). Around 157 bp, an intergenic spacer was identified. The overall GC-skews and AT-skews of the H-strand mitogenome were -0.35 and 0.079, respectively, revealing that the H-strand consisted of equal amounts of A and T and that the overall nucleotide composition was C skewed. All tRNA genes exhibited cloverleaf secondary structures, while the secondary structure of tRNA lacked a discernible dihydrouridine stem. The phylogenetic analysis of available mitogenomes of Anabantiformes revealed a sister group relationship between Pristolepididae and Channidae. The whole mitogenome of will form a molecular resource for further taxonomic and conservation studies on this endemic freshwater fish.
淡水叶鱼属于攀鲈科，仅分布于印度喀拉拉邦的潘巴和查拉库迪河。本研究对攀鲈科的淡水叶鱼进行了全线粒体基因组测序，并分析了其在攀鲈目鱼类中的系统发育关系。该线粒体基因组全长 16622bp，包含 13 个蛋白编码基因、2 个 rRNA 基因、22 个转移 RNA（tRNA）基因和一个非编码控制区。所有的蛋白编码基因、tRNA 和 rRNA 都位于重链上，除了 nad6 和 8 个 tRNA（谷氨酰胺、丙氨酸、天冬酰胺、半胱氨酸、酪氨酸、丝氨酸、谷氨酸和脯氨酸）是从 L 链转录的。该基因组在 atp8 和 atp6（2bp）、nad4 和 nad4l（2bp）、tRNA 和 tRNA（1bp）、tRNA 和 tRNA（1bp）之间存在重叠。在 157bp 左右，鉴定出一个基因间间隔区。H 链线粒体基因组的整体 GC 倾斜和 AT 倾斜分别为-0.35 和 0.079，表明 H 链含有等量的 A 和 T，整体核苷酸组成呈 C 倾斜。所有 tRNA 基因都表现出三叶草二级结构，而 tRNA 的二级结构缺乏可识别的双氢尿嘧啶茎。对已有的攀鲈目鱼类线粒体基因组的系统发育分析表明，攀鲈科与攀鲈科具有姐妹群关系。淡水叶鱼的全线粒体基因组将为进一步研究这种特有淡水鱼类的分类学和保护生物学提供分子资源。
