通过胚胎干细胞（ES）细胞显微注射生成种间鼠-大鼠嵌合体胚胎。
Generation of interspecies mouse-rat chimeric embryos by embryonic stem (ES) cell microinjection.
机构信息
Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Institute of Anatomy, Medical Faculty Carl-Gustav-Carus, Technische Universität Dresden, 01307 Dresden, Germany.
出版信息
STAR Protoc. 2021 Jun 15;2(2):100494. doi: 10.1016/j.xpro.2021.100494. eCollection 2021 Jun 18.
Interspecies chimerism is a useful tool to study interactions between cells of different genetic makeup in order to elucidate the mechanisms underlying non-cell-autonomous processes, including evolutionary events. However, generating interspecies chimeras with high efficiency and chimerism level remains challenging. Here, we describe a protocol for generating chimeras between mouse and rat. Donor embryonic stem cells of one species are microinjected into early embryos of the other species (recipient), which are implanted into host foster mothers of the recipient species. For complete details on the use and execution of this protocol, please refer to Stepien et al. (2020).
种间嵌合体是一种有用的工具，可用于研究不同遗传背景的细胞之间的相互作用，以阐明非细胞自主过程（包括进化事件）的机制。然而，高效且高水平地产生种间嵌合体仍然具有挑战性。在这里，我们描述了一种在小鼠和大鼠之间产生嵌合体的方案。将一种物种的供体胚胎干细胞微注射到另一种物种（受体）的早期胚胎中，然后将其植入受体物种的宿主代孕母亲体内。有关此方案的使用和执行的完整详细信息，请参阅 Stepien 等人（2020 年）。
Zotero 插件