Hirano T, Miyajima H, Taniguchi O, Ueda A, Takai S, Hashimoto H, Hirose S, Okumura K
Department of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, Japan.
Jpn J Med. 1988 May;27(2):167-71. doi: 10.2169/internalmedicine1962.27.167.
A high incidence of antibody to asialo GM1 was observed in the sera from the patients with Systemic Lupus Erythematosus (SLE) and Behçet's disease with neurological manifestations, using Enzyme Linked Immunosorbent Assay (ELISA), and Thin-layer Chromatography (TLC) immunostaining. The sera from 60 out of 102 cases of SLE with neurological disorders and 6 out of 10 patients with neuro Behçet's disease showed antibody activity against asialo GM1 but not against the asialo GM2, GM1 and galactocerebroside. In 7 out of 123 cases SLE having a history without neurological manifestations and 1 out of 19 Behçet's patients without neurological disorders, antiasialo GM1 antibody could be detectable. However, sera from the patients with other autoimmune diseases, such as RA (60 cases) and P S S (32 cases) or from normal subjects did not show any antibody activity against asialo GM1. Antiasialo GM1 antibody activity presents in both IgM and IgG immunoglobulin classes by class specific ELISA and TLC immunostaining. These studies suggest that detection of antiasialo GM1 antibody may be useful in clinical diagnosis and these autoantibody plays a important role in the pathogenesis of neurological manifestations accompanying SLE and Neuro-Behçet's disease.
采用酶联免疫吸附测定法（ELISA）和薄层色谱（TLC）免疫染色法，在系统性红斑狼疮（SLE）患者以及伴有神经症状的白塞病患者的血清中，观察到抗唾液酸GM1抗体的高发生率。102例伴有神经障碍的SLE患者中有60例，10例神经白塞病患者中有6例的血清显示出针对唾液酸GM1的抗体活性，但对唾液酸GM2、GM1和半乳糖脑苷脂无抗体活性。在123例无神经症状病史的SLE患者中有7例，19例无神经障碍的白塞病患者中有1例可检测到抗唾液酸GM1抗体。然而，其他自身免疫性疾病患者（如60例类风湿关节炎患者和32例原发性干燥综合征患者）或正常受试者的血清未显示出针对唾液酸GM1的任何抗体活性。通过类特异性ELISA和TLC免疫染色法发现，抗唾液酸GM1抗体活性同时存在于IgM和IgG免疫球蛋白类别中。这些研究表明，抗唾液酸GM1抗体的检测可能有助于临床诊断，并且这些自身抗体在伴有SLE和神经白塞病的神经症状的发病机制中起重要作用。
