Department of Biomedical Engineering, The University of Texas at San Antonio, San Antonio, Texas, USA.
Department of Chemical and Petroleum Engineering, Bioengineering Graduate Program, School of Engineering, University of Kansas, Lawrence, Kansas, USA.
J Biomed Mater Res A. 2021 Dec;109(12):2597-2610. doi: 10.1002/jbm.a.37253. Epub 2021 Jun 29.
Hydrogels can be fabricated and designed to exert direct control over stem cells' adhesion and differentiation. In this study, we have investigated the use of polydopamine (pDA)-treatment as a binding platform for bioactive compounds to create a versatile gelatin-alginate (Gel-Alg) hydrogel for tissue engineering applications. Precisely, pDA was used to modify the surface properties of the hydrogel and better control the adhesion and osteogenic differentiation of human adipose-derived stem cells (hASCs). pDA enabled the adsorption of different types of bioactive molecules, including a model osteoinductive drug (dexamethasone) as well as a model pro-angiogenic peptide (QK). The pDA treatment efficiently retained the drug and the peptide compared to the untreated hydrogel and proved to be effective in controlling the morphology, cell area, and osteogenic differentiation of hASCs. Overall, the findings of this study confirm the efficacy of pDA treatment as a valuable strategy to modulate the biological properties of biocompatible Gel-Alg hydrogels and further extend their value in regenerative medicine.
水凝胶可以被制造和设计，以直接控制干细胞的黏附和分化。在这项研究中，我们研究了使用聚多巴胺（pDA）处理作为生物活性化合物的结合平台，以创建一种用于组织工程应用的通用明胶-海藻酸盐（Gel-Alg）水凝胶。具体来说，pDA 被用于修饰水凝胶的表面性质，并更好地控制人脂肪来源干细胞（hASC）的黏附和成骨分化。pDA 能够吸附不同类型的生物活性分子，包括模型成骨诱导药物（地塞米松）和模型促血管生成肽（QK）。与未处理的水凝胶相比，pDA 处理有效地保留了药物和肽，并且被证明能够有效地控制 hASC 的形态、细胞面积和成骨分化。总的来说，这项研究的结果证实了 pDA 处理作为一种有价值的策略，可调节生物相容性 Gel-Alg 水凝胶的生物学特性，并进一步扩展其在再生医学中的价值。
