制备具有聚多巴胺修饰的双交联互穿聚合物网络（IPN）水凝胶表面以调控脂肪来源干细胞的成骨分化。
Fabrication of a Double-Cross-Linked Interpenetrating Polymeric Network (IPN) Hydrogel Surface Modified with Polydopamine to Modulate the Osteogenic Differentiation of Adipose-Derived Stem Cells.
机构信息
Department of Chemical and Petroleum Engineering, BioIntel Research Laboratory , University of Kansas , Lawrence , Kansas 66045 , United States.
Biomechanics Research Centre (BMEC), Mechanical and Biomedical Engineering, College of Engineering and Informatics , National University of Ireland , Galway , H91 TK33 Ireland.
出版信息
ACS Appl Mater Interfaces. 2018 Aug 1;10(30):24955-24962. doi: 10.1021/acsami.8b05200. Epub 2018 Jul 9.
Abstract
Hydrogel surface properties can be modified to form bioactive interfaces to modulate the osteogenic differentiation of stem cells. In this work, a hydrogel made of gelatin methacrylamide (GelMA) and alginate was designed and tested as a scaffold to control stem-cell osteogenic differentiation. The hydrogel's surface was treated with polydopamine (pDA) to create an adhesive layer for the adsorption of the osteoinductive drug dexamethasone (Dex). The presence of the pDA coating enhanced Dex adsorption and retention over 21 days. This effect resulted in a delay in the osteogenic differentiation of hASCs cultured on the hydrogel treated with a pDA layer.
摘要
水凝胶的表面性质可以被修饰以形成生物活性界面，从而调节干细胞的成骨分化。在这项工作中，设计并测试了一种由明胶甲基丙烯酰胺（GelMA）和藻酸盐组成的水凝胶作为支架来控制干细胞的成骨分化。水凝胶的表面用聚多巴胺（pDA）处理，以形成用于吸附成骨诱导药物地塞米松（Dex）的黏附层。pDA 涂层的存在增强了 Dex 在 21 天内的吸附和保留。这种效果导致在经过 pDA 层处理的水凝胶上培养的 hASC 的成骨分化延迟。
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