School of Basic Medicine & Public Health, Jinan University, Guangzhou, 510630, China.
Department of Stomatology, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510630, China.
Nanomedicine (Lond). 2021 Aug;16(18):1567-1579. doi: 10.2217/nnm-2021-0025. Epub 2021 Jun 30.
Bone mesenchymal stem cell-derived exosomes (Exos) have been shown to exert therapeutic effects in spinal cord injury (SCI). In this study, we aimed to apply bioengineering approaches to promote Exo retention and their sustained release for SCI repair. 3D gelatin methacrylate hydrogel (GelMA) was used as a transplanted Exo delivery system (GelMA-Exos). The viability, proliferation, and differentiation of neural stem cells cultured on hydrogel were assessed. Further, GelMA-Exos was injected into the damaged lesions to assess its repair potential. GelMA hydrogel enhanced the retention of Exos, which promoted the neuronal differentiation and extension . Furthermore, GelMA-Exos promoted neurogenesis and attenuated glial scars in the damaged lesions. The injectable Exo-loaded 3D hydrogel induced neurological functional recovery post SCI.
骨髓间充质干细胞来源的外泌体 (Exos) 已被证明在脊髓损伤 (SCI) 中具有治疗作用。在这项研究中，我们旨在应用生物工程方法来促进外泌体的保留和持续释放，以修复 SCI。3D 明胶甲基丙烯酸盐水凝胶 (GelMA) 被用作外泌体输送系统 (GelMA-Exos)。评估了在水凝胶上培养的神经干细胞的活力、增殖和分化。进一步将 GelMA-Exos 注射到损伤部位，以评估其修复潜力。GelMA 水凝胶增强了外泌体的保留，促进了神经元的分化和延伸。此外，GelMA-Exos 促进了损伤部位的神经发生并减轻了胶质瘢痕形成。可注射的负载外泌体的 3D 水凝胶在 SCI 后诱导了神经功能的恢复。
