Department of Materials, Imperial College London, London, UK.
Nanoscale. 2021 Jul 15;13(27):11921-11931. doi: 10.1039/d1nr02584h.
Novel methods for introducing chemical and biological functionality to the surface of gold nanoparticles serve to increase the utility of this class of nanomaterials across a range of applications. To date, methods for functionalising gold surfaces have relied upon uncontrollable non-specific adsorption, bespoke chemical linkers, or non-generalisable protein-protein interactions. Herein we report a versatile method for introducing functionality to gold nanoparticles by exploiting the strong interaction between chemically functionalised bovine serum albumin (f-BSA) and citrate-capped gold nanoparticles (AuNPs). We establish the generalisability of the method by introducing a variety of functionalities to gold nanoparticles using cheap, commercially available chemical linkers. The utility of this approach is further demonstrated through the conjugation of the monoclonal antibody Ontruzant to f-BSA-AuNPs using inverse electron-demand Diels-Alder (iEDDA) click chemistry, a hitherto unexplored chemistry for AuNP-IgG conjugation. Finally, we show that the AuNP-Ontruzant particles generated via f-BSA-AuNPs have a greater affinity for their target in a lateral flow format when compared to conventional physisorption, highlighting the potential of this technology for producing sensitive diagnostic tests.
新型方法可将化学和生物官能团引入金纳米粒子的表面，从而提高此类纳米材料在一系列应用中的实用性。迄今为止，对金表面进行功能化的方法依赖于不可控的非特异性吸附、定制的化学连接子或不可推广的蛋白质-蛋白质相互作用。在此，我们报告了一种通过利用化学官能化牛血清白蛋白（f-BSA）和柠檬酸盐封端的金纳米粒子（AuNPs）之间的强相互作用向金纳米粒子引入功能的通用方法。我们通过使用廉价的商业化学连接子将各种官能团引入金纳米粒子，从而确立了该方法的通用性。通过使用反向电子需求 Diels-Alder (iEDDA)点击化学将单克隆抗体 Ontruzant 连接到 f-BSA-AuNPs 上，进一步证明了该方法的实用性，这是 AuNP-IgG 连接中迄今尚未探索的化学方法。最后，我们表明，与传统的物理吸附相比，通过 f-BSA-AuNPs 生成的 AuNP-Ontruzant 颗粒在横向流动格式中对其靶标具有更高的亲和力，突出了该技术在生产敏感诊断测试方面的潜力。
