Smith Jeffrey S, Hanseler Haley, Welle John, Rattray Rogan, Campbell Mary, Brotherton Tacy, Moudgil Tarsem, Pack Thomas F, Wegmann Keith, Jensen Shawn, Jin Justin, Bifulco Carlo B, Prahl Scott A, Fox Bernard A, Stucky Nicholas L
Providence Portland Medical Center, Department of Medicine, Portland, Oregon, USA.
OHSU Medical School, Portland, Oregon, USA.
J Clin Transl Sci. 2020 Jun 11;5(1):e10. doi: 10.1017/cts.2020.494.
The COVID-19 pandemic has created a high demand on personal protective equipment, including disposable N95 masks. Given the need for mask reuse, we tested the feasibility of vaporized hydrogen peroxide (VHP), ultraviolet light (UV), and ethanol decontamination strategies on N95 mask integrity and the ability to remove the infectious potential of SARS-CoV-2.
Disposable N95 masks, including medical grade (1860, 1870+) and industrial grade (8511) masks, were treated by VHP, UV, and ethanol decontamination. Mask degradation was tested using a quantitative respirator fit testing. Pooled clinical samples of SARS-CoV-2 were applied to mask samples, treated, and then either sent immediately for real-time reverse transcriptase-polymerase chain reaction (RT-PCR) or incubated with Vero E6 cells to assess for virucidal effect.
Both ethanol and UV decontamination showed functional degradation to different degrees while VHP treatment showed no significant change after two treatments. We also report a single SARS-CoV-2 virucidal experiment using Vero E6 cell infection in which only ethanol treatment eliminated detectable SARS-CoV-2 RNA.
We hope our data will guide further research for evidenced-based decisions for disposable N95 mask reuse and help protect caregivers from SARS-CoV-2 and other pathogens.
新型冠状病毒肺炎（COVID-19）大流行对个人防护装备产生了巨大需求，包括一次性N95口罩。鉴于口罩重复使用的需求，我们测试了汽化过氧化氢（VHP）、紫外线（UV）和乙醇消毒策略对N95口罩完整性以及去除严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染潜力的可行性。
一次性N95口罩，包括医用级（1860、1870+）和工业级（8511）口罩，采用VHP、UV和乙醇进行消毒处理。使用定量呼吸器适配测试来检测口罩的降解情况。将SARS-CoV-2的混合临床样本应用于口罩样本，进行处理，然后立即送去进行实时逆转录聚合酶链反应（RT-PCR），或者与非洲绿猴肾细胞（Vero E6）共孵育以评估杀毒效果。
乙醇和UV消毒均显示出不同程度的功能降解，而VHP处理在两次处理后未显示出显著变化。我们还报告了一项使用Vero E6细胞感染的单一SARS-CoV-2杀毒实验，其中只有乙醇处理消除了可检测到的SARS-CoV-2 RNA。
我们希望我们的数据将为一次性N95口罩重复使用的循证决策指导进一步研究，并帮助保护医护人员免受SARS-CoV-2和其他病原体的侵害。
