Argonaute-微 RNA-SGS3 复合物引起的核糖体停滞调控植物中次级 siRNA 的产生。
Ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates the production of secondary siRNAs in plants.
机构信息
Laboratory of RNA Function, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan; JST, PRESTO, Kawaguchi, Saitama 332-0012, Japan.
Laboratory of RNA Function, Institute for Quantitative Biosciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
出版信息
Cell Rep. 2021 Jun 29;35(13):109300. doi: 10.1016/j.celrep.2021.109300.
The path of ribosomes on mRNAs can be impeded by various obstacles. One such example is halting of ribosome movement by microRNAs, but the exact mechanism and physiological role remain unclear. Here, we find that ribosome stalling caused by the Argonaute-microRNA-SGS3 complex regulates production of secondary small interfering RNAs (siRNAs) in plants. We show that the double-stranded RNA-binding protein SGS3 interacts directly with the 3' end of the microRNA in an Argonaute protein, resulting in ribosome stalling. Importantly, microRNA-mediated ribosome stalling correlates positively with efficient production of secondary siRNAs from target mRNAs. Our results illustrate a role of paused ribosomes in regulation of small RNA function that may have broad biological implications across the plant kingdom.
核糖体在 mRNA 上的行进路径可能会受到各种障碍物的阻碍。例如，microRNA 会阻止核糖体的运动，但确切的机制和生理作用尚不清楚。在这里，我们发现 Argonaute-microRNA-SGS3 复合物引起的核糖体停滞会调节植物中次级小干扰 RNA（siRNA）的产生。我们表明，双链 RNA 结合蛋白 SGS3 与 Argonaute 蛋白中的 microRNA 的 3' 端直接相互作用，导致核糖体停滞。重要的是，microRNA 介导的核糖体停滞与靶 mRNA 产生高效次级 siRNA 呈正相关。我们的研究结果说明了暂停核糖体在调节小 RNA 功能方面的作用，这可能在整个植物界具有广泛的生物学意义。
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