• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

ZmNAC080308 非编码区的自然变异有助于玉米在干旱胁迫下保持产量。

Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize.

机构信息

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.

International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico.

出版信息

BMC Plant Biol. 2021 Jun 30;21(1):305. doi: 10.1186/s12870-021-03072-9.

DOI:10.1186/s12870-021-03072-9
PMID:34193036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8243440/
Abstract

BACKGROUND

Natural variations derived from both evolutionary selection and genetic recombination, presume to have important functions to respond to various abiotic stresses, which could be used to improve drought tolerance via genomic selection.

RESULTS

In the present study, the NAC-encoding gene of ZmNAC080308 was cloned and sequenced in 199 inbred lines in maize. Phylogenetic analysis showed that ZmNAC080308 is closely clusteredinto the same group with other well-known NAC genes responding to improve drought tolerance. In total, 86 SNPs and 47 InDels were identified in the generic region of ZmNAC080308, 19 of these variations were associated with GY (grain yield) in different environments. Nine variations in the 5'-UTR region of ZmNAC080308 are closely linked, they might regulate the gene expression and respond to improve GY under drought condition via Sp1-mediated transactivation. Two haplotypes (Hap1 and Hap2) identified in the, 5'-UTR region using the nine variations, and Hap2 containing insertion variants, exhibited 15.47 % higher GY under drought stress condition. Further, a functional marker was developed to predict the drought stress tolerance in a US maize inbred line panel. Lines carrying Hap2 exhibited > 10 % higher GY than those carrying Hap1 under drought stress condition. In Arabidopsis, overexpression ZmNAC080308 enhanced drought tolerance.

CONCLUSIONS

ZmNAC080308 is an important gene responding to drought tolerance, a functional marker is developed for improving maize drought tolerance by selecting this gene.

摘要

背景

自然变异源于进化选择和遗传重组，据推测对各种非生物胁迫具有重要功能，可用于通过基因组选择提高耐旱性。

结果

本研究在 199 个玉米自交系中克隆和测序了 NAC 编码基因 ZmNAC080308。系统发育分析表明，ZmNAC080308与其他响应提高耐旱性的知名 NAC 基因密切聚类到同一组。在 ZmNAC080308 的基因区域共鉴定出 86 个 SNP 和 47 个 InDels，其中 19 个变异与不同环境下的 GY（粒重）有关。ZmNAC080308 5'-UTR 区域的 9 个变异紧密连锁，它们可能通过 Sp1 介导的反式激活调节基因表达并响应提高干旱条件下的 GY。在 5'-UTR 区域使用这 9 个变异鉴定出两个单倍型（Hap1 和 Hap2），并且包含插入变异的 Hap2 表现出干旱胁迫条件下 15.47%更高的 GY。此外，开发了一个功能标记来预测美国玉米自交系群体中的干旱胁迫耐受性。携带 Hap2 的系在干旱胁迫条件下表现出比携带 Hap1 的系高 10%以上的 GY。在拟南芥中，过表达 ZmNAC080308 增强了耐旱性。

结论

ZmNAC080308 是一个响应耐旱性的重要基因，开发了一个功能标记用于通过选择该基因来提高玉米的耐旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/7800fbd86516/12870_2021_3072_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/b145c69ab615/12870_2021_3072_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/d8e9363ff565/12870_2021_3072_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/b547d600ad60/12870_2021_3072_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/da9a438bd00f/12870_2021_3072_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/d5805df0e8ea/12870_2021_3072_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/a36172f3db9e/12870_2021_3072_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/7800fbd86516/12870_2021_3072_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/b145c69ab615/12870_2021_3072_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/d8e9363ff565/12870_2021_3072_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/b547d600ad60/12870_2021_3072_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/da9a438bd00f/12870_2021_3072_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/d5805df0e8ea/12870_2021_3072_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/a36172f3db9e/12870_2021_3072_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/049c/8243440/7800fbd86516/12870_2021_3072_Fig7_HTML.jpg

相似文献

1
Natural variations in the non-coding region of ZmNAC080308 contributes maintaining grain yield under drought stress in maize.ZmNAC080308 非编码区的自然变异有助于玉米在干旱胁迫下保持产量。
BMC Plant Biol. 2021 Jun 30;21(1):305. doi: 10.1186/s12870-021-03072-9.
2
ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis.ZmNAC55，一种玉米应激响应的 NAC 转录因子，可赋予转基因拟南芥抗旱性。
Plant Physiol Biochem. 2016 Aug;105:55-66. doi: 10.1016/j.plaphy.2016.04.018. Epub 2016 Apr 11.
3
Genome-Wide Analysis of TCP Family Genes in L. Identified a Role for in Drought Tolerance.对 L. 中 TCP 家族基因的全基因组分析表明 基因在耐旱性中起作用。
Int J Mol Sci. 2019 Jun 5;20(11):2762. doi: 10.3390/ijms20112762.
4
The Maize WRKY Transcription Factor ZmWRKY40 Confers Drought Resistance in Transgenic .玉米 WRKY 转录因子 ZmWRKY40 赋予转基因. 的抗旱性。
Int J Mol Sci. 2018 Aug 30;19(9):2580. doi: 10.3390/ijms19092580.
5
Genome-wide analysis of ZmDREB genes and their association with natural variation in drought tolerance at seedling stage of Zea mays L.玉米ZmDREB 基因的全基因组分析及其与玉米幼苗期抗旱性自然变异的关联
PLoS Genet. 2013;9(9):e1003790. doi: 10.1371/journal.pgen.1003790. Epub 2013 Sep 26.
6
A novel NAC transcription factor ZmNAC55 negatively regulates drought stress in Zea mays.一个新的 NAC 转录因子 ZmNAC55 负调控玉米中的干旱胁迫。
Plant Physiol Biochem. 2024 Sep;214:108938. doi: 10.1016/j.plaphy.2024.108938. Epub 2024 Jul 14.
7
A maize stress-responsive NAC transcription factor, ZmSNAC1, confers enhanced tolerance to dehydration in transgenic Arabidopsis.一个玉米胁迫响应的 NAC 转录因子，ZmSNAC1，赋予转基因拟南芥增强的脱水耐性。
Plant Cell Rep. 2012 Sep;31(9):1701-11. doi: 10.1007/s00299-012-1284-2. Epub 2012 May 18.
8
Deletion of an Endoplasmic Reticulum Stress Response Element in a ZmPP2C-A Gene Facilitates Drought Tolerance of Maize Seedlings.ZmPP2C-A 基因中内质网应激反应元件的缺失促进了玉米幼苗的耐旱性。
Mol Plant. 2017 Mar 6;10(3):456-469. doi: 10.1016/j.molp.2016.10.003. Epub 2016 Oct 13.
9
A large-scale circular RNA profiling reveals universal molecular mechanisms responsive to drought stress in maize and Arabidopsis.大规模环状 RNA 分析揭示了玉米和拟南芥响应干旱胁迫的普遍分子机制。
Plant J. 2019 May;98(4):697-713. doi: 10.1111/tpj.14267. Epub 2019 Mar 5.
10
Regulatory mechanisms used by ZmMYB39 to enhance drought tolerance in maize (Zea mays) seedlings.ZmMYB39 增强玉米幼苗抗旱性的调控机制。
Plant Physiol Biochem. 2024 Jun;211:108696. doi: 10.1016/j.plaphy.2024.108696. Epub 2024 May 3.

引用本文的文献

1
Genetic Dissection of Drought Tolerance in Maize Through GWAS of Agronomic Traits, Stress Tolerance Indices, and Phenotypic Plasticity.通过对农艺性状、胁迫耐受性指标和表型可塑性进行全基因组关联研究对玉米耐旱性进行遗传剖析
Int J Mol Sci. 2025 Jun 29;26(13):6285. doi: 10.3390/ijms26136285.
2
Identification of , a Maize NAC Family Transcription Factor with Multiple Transcripts Conferring Drought Tolerance in .一种具有多种转录本并赋予玉米耐旱性的玉米NAC家族转录因子的鉴定。
Plants (Basel). 2024 Dec 24;14(1):12. doi: 10.3390/plants14010012.
3
Meta-Quantitative Trait Loci Analysis and Candidate Gene Mining for Drought Tolerance-Associated Traits in Maize ( L.).

本文引用的文献

1
CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP.系统发育树的置信区间：一种使用自展法的方法。
Evolution. 1985 Jul;39(4):783-791. doi: 10.1111/j.1558-5646.1985.tb00420.x.
2
Natural variation for gene expression responses to abiotic stress in maize.玉米中基因表达对非生物胁迫响应的自然变异。
Plant J. 2017 Feb;89(4):706-717. doi: 10.1111/tpj.13414. Epub 2017 Feb 11.
3
Deletion of an Endoplasmic Reticulum Stress Response Element in a ZmPP2C-A Gene Facilitates Drought Tolerance of Maize Seedlings.ZmPP2C-A 基因中内质网应激反应元件的缺失促进了玉米幼苗的耐旱性。
玉米（L.）耐旱相关性状的元定量性状位点分析及候选基因挖掘
Int J Mol Sci. 2024 Apr 12;25(8):4295. doi: 10.3390/ijms25084295.
4
Maize ZmLAZ1-3 gene negatively regulates drought tolerance in transgenic Arabidopsis.玉米 ZmLAZ1-3 基因负调控转基因拟南芥的耐旱性。
BMC Plant Biol. 2024 Apr 5;24(1):246. doi: 10.1186/s12870-024-04923-x.
5
Radiation Hormesis in Barley Manifests as Changes in Growth Dynamics Coordinated with the Expression of , , and .大麦中的辐射兴奋效应表现为生长动态的变化，这些变化与 、 、 和 的表达相协调。
Int J Mol Sci. 2024 Jan 12;25(2):974. doi: 10.3390/ijms25020974.
6
Sequencing and Phylogenetic Analysis of the Chloroplast Genome of Three Apricot Species.三种杏属植物叶绿体基因组的测序和系统发育分析。
Genes (Basel). 2023 Oct 18;14(10):1959. doi: 10.3390/genes14101959.
7
Molecular mechanisms of drought resistance using genome-wide association mapping in maize (Zea mays L.).利用全基因组关联图谱分析玉米抗旱性的分子机制。
BMC Plant Biol. 2023 Oct 6;23(1):468. doi: 10.1186/s12870-023-04489-0.
8
Transcriptome Analyses Show Changes in Gene Expression Triggered by a 31-bp InDel within 5'UTR in Rice Panicle.转录组分析表明，水稻穗内 5'UTR 内的 31-bp 缺失导致基因表达发生变化。
Int J Mol Sci. 2023 Jun 26;24(13):10640. doi: 10.3390/ijms241310640.
9
Transcription Factor ZmNAC20 Improves Drought Resistance by Promoting Stomatal Closure and Activating Expression of Stress-Responsive Genes in Maize.转录因子 ZmNAC20 通过促进气孔关闭和激活玉米中应激响应基因的表达来提高耐旱性。
Int J Mol Sci. 2023 Mar 1;24(5):4712. doi: 10.3390/ijms24054712.
10
Improving drought tolerance in maize: Tools and techniques.提高玉米的耐旱性：工具与技术
Front Genet. 2022 Oct 28;13:1001001. doi: 10.3389/fgene.2022.1001001. eCollection 2022.
Mol Plant. 2017 Mar 6;10(3):456-469. doi: 10.1016/j.molp.2016.10.003. Epub 2016 Oct 13.
4
Genetic variation in ZmVPP1 contributes to drought tolerance in maize seedlings.ZmVPP1 中的遗传变异有助于玉米幼苗的耐旱性。
Nat Genet. 2016 Oct;48(10):1233-41. doi: 10.1038/ng.3636. Epub 2016 Aug 15.
5
ZmNAC55, a maize stress-responsive NAC transcription factor, confers drought resistance in transgenic Arabidopsis.ZmNAC55，一种玉米应激响应的 NAC 转录因子，可赋予转基因拟南芥抗旱性。
Plant Physiol Biochem. 2016 Aug;105:55-66. doi: 10.1016/j.plaphy.2016.04.018. Epub 2016 Apr 11.
6
MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7：适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.
7
NAC transcription factors in plant multiple abiotic stress responses: progress and prospects.植物多重非生物胁迫响应中的NAC转录因子：研究进展与展望
Front Plant Sci. 2015 Oct 29;6:902. doi: 10.3389/fpls.2015.00902. eCollection 2015.
8
TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis.TaNAC29是一种来自小麦的NAC转录因子，可增强转基因拟南芥的耐盐性和耐旱性。
BMC Plant Biol. 2015 Nov 4;15:268. doi: 10.1186/s12870-015-0644-9.
9
A transposable element in a NAC gene is associated with drought tolerance in maize seedlings.NAC基因中的一个转座元件与玉米幼苗的耐旱性相关。
Nat Commun. 2015 Sep 21;6:8326. doi: 10.1038/ncomms9326.
10
Functional characterization and reconstitution of ABA signaling components using transient gene expression in rice protoplasts.利用水稻原生质体中的瞬时基因表达对脱落酸信号转导组分进行功能表征与重组
Front Plant Sci. 2015 Aug 5;6:614. doi: 10.3389/fpls.2015.00614. eCollection 2015.