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玉米 ZmLAZ1-3 基因负调控转基因拟南芥的耐旱性。

Maize ZmLAZ1-3 gene negatively regulates drought tolerance in transgenic Arabidopsis.

机构信息

Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, People's Republic of China.

College of Food and Biological Engineering, Chengdu University, Chengdu, 610106, People's Republic of China.

出版信息

BMC Plant Biol. 2024 Apr 5;24(1):246. doi: 10.1186/s12870-024-04923-x.

DOI:10.1186/s12870-024-04923-x
PMID:38575869
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10996212/
Abstract

BACKGROUND

Molecular mechanisms in response to drought stress are important for the genetic improvement of maize. In our previous study, nine ZmLAZ1 members were identified in the maize genome, but the function of ZmLAZ1 was largely unknown.

RESULTS

The ZmLAZ1-3 gene was cloned from B73, and its drought-tolerant function was elucidated by expression analysis in transgenic Arabidopsis. The expression of ZmLAZ1-3 was upregulated by drought stress in different maize inbred lines. The driving activity of the ZmLAZ1-3 promoter was induced by drought stress and related to the abiotic stress-responsive elements such as MYB, MBS, and MYC. The results of subcellular localization indicated that the ZmLAZ1-3 protein localized on the plasma membrane and chloroplast. The ectopic expression of the ZmLAZ1-3 gene in Arabidopsis significantly reduced germination ratio and root length, decreased biomass, and relative water content, but increased relative electrical conductivity and malondialdehyde content under drought stress. Moreover, transcriptomics analysis showed that the differentially expressed genes between the transgenic lines and wild-type were mainly associated with response to abiotic stress and biotic stimulus, and related to pathways of hormone signal transduction, phenylpropanoid biosynthesis, mitogen-activated protein kinase signaling, and plant-pathogen interaction.

CONCLUSION

The study suggests that the ZmLAZ1-3 gene is a negative regulator in regulating drought tolerance and can be used to improve maize drought tolerance via its silencing or knockout.

摘要

背景

响应干旱胁迫的分子机制对于玉米的遗传改良很重要。在我们之前的研究中，在玉米基因组中鉴定了九个 ZmLAZ1 成员，但 ZmLAZ1 的功能很大程度上是未知的。

结果

从 B73 中克隆了 ZmLAZ1-3 基因，并通过在转基因拟南芥中的表达分析阐明了其耐旱功能。不同玉米自交系中 ZmLAZ1-3 的表达受干旱胁迫上调。ZmLAZ1-3 启动子的驱动活性受干旱胁迫诱导，并与 MYB、MBS 和 MYC 等非生物胁迫响应元件有关。亚细胞定位结果表明，ZmLAZ1-3 蛋白定位于质膜和叶绿体上。ZmLAZ1-3 基因在拟南芥中的异位表达显著降低了发芽率和根长，降低了生物量和相对含水量，但在干旱胁迫下相对电导率和丙二醛含量增加。此外，转录组学分析表明，转基因株系与野生型之间差异表达的基因主要与非生物胁迫和生物刺激的反应有关，与激素信号转导、苯丙烷生物合成、丝裂原活化蛋白激酶信号转导和植物-病原体相互作用途径有关。

结论

该研究表明，ZmLAZ1-3 基因是调节耐旱性的负调控因子，可通过其沉默或敲除来提高玉米的耐旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/c93f79b9a0b4/12870_2024_4923_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/3d8667a29f1b/12870_2024_4923_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/502ec61bed45/12870_2024_4923_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/a0e88c6d6db7/12870_2024_4923_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/e534c7ea4674/12870_2024_4923_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/c93f79b9a0b4/12870_2024_4923_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/3d8667a29f1b/12870_2024_4923_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/8b5a89827e8f/12870_2024_4923_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/9a6afe74e02d/12870_2024_4923_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/502ec61bed45/12870_2024_4923_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/a0e88c6d6db7/12870_2024_4923_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/e534c7ea4674/12870_2024_4923_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/68c9/10996212/c93f79b9a0b4/12870_2024_4923_Fig7_HTML.jpg

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