Yusuf Babalola Ola, Yakubu Musa Toyin, Akanji Musbau Adewumi
Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria.
Department of Chemical Sciences, College of Natural and Applied Sciences, Summit University Offa, Nigeria.
J Tradit Complement Med. 2021 Jan 21;11(4):336-342. doi: 10.1016/j.jtcme.2021.01.005. eCollection 2021 Jul.
Diabetes, with hyperglycaemia as hallmark, is a global crisis that reduces the antioxidant status and produces complications when poorly managed. The development of complications can be indicated by inflammation, lipid peroxidation and the accumulation of glycation adducts. Thus, the attenuation of hyperglycaemia and boosting of antioxidants status is key in ameliorating markers of diabetes complications. This work evaluated the potency of stem bark on some markers of diabetes complications.
A total of 100 female rats (180.80 ± 8.50 g) were assigned into ten groups of ten animals each; control received 1.0 ml of distilled water while those in groups DC, RD, F1, F3, F4, F5, F7, F9, F10 were induced into diabetes by intraperitoneal administration of 120 mg/kg body weight of alloxan and were orally administered distilled water, glibenclamide, 2.5 mg/kg of the chromatographic fractions 1, 3, 4, 5, 7, 9, and 10 respectively, once daily for 14 days. F7 was profiled for its bioactive constituents and the pancreas histology of the rats were examined.
Chromatographic fractions F5 and F7 significantly decreased fasting blood glucose, glycosylated haemoglobin, C-reactive protein, total cholesterol, triglycerides, atherogenic index, malondialdehyde while insulin, high density lipoprotein, catalase, superoxide dismutase activities significantly increased. Fraction F7 revealed eight compounds and restored the distorted pancreas.
Fraction F7 ameliorated the markers of diabetes complications considered in this study better than F5, restored the compromised pancreas and can be explored as lead candidate for production of drug for the management of diabetes.
糖尿病以高血糖为特征，是一场全球性危机，会降低抗氧化状态，管理不善时会引发并发症。并发症的发展可通过炎症、脂质过氧化和糖化加合物的积累来表明。因此，减轻高血糖和提高抗氧化状态是改善糖尿病并发症指标的关键。本研究评估了茎皮对糖尿病并发症某些指标的作用效果。
总共100只雌性大鼠（体重180.80±8.50克）被分为十组，每组十只；对照组给予1.0毫升蒸馏水，而DC、RD、F1、F3、F4、F5、F7、F9、F10组的大鼠通过腹腔注射120毫克/千克体重的四氧嘧啶诱导成糖尿病，并分别每日口服蒸馏水、格列本脲、2.5毫克/千克的色谱馏分1、3、4、5、7、9和10，持续14天。对F7进行生物活性成分分析，并检查大鼠的胰腺组织学。
色谱馏分F5和F7显著降低空腹血糖、糖化血红蛋白、C反应蛋白、总胆固醇、甘油三酯、动脉粥样硬化指数、丙二醛，而胰岛素、高密度脂蛋白、过氧化氢酶、超氧化物歧化酶活性显著增加。馏分F7鉴定出八种化合物，并使变形的胰腺恢复正常。
在本研究中，馏分F7比F5能更好地改善糖尿病并发症指标，恢复受损的胰腺，可作为开发糖尿病治疗药物的主要候选物进行探索。
