Ojo Oluwafemi Adeleke, Ajiboye Basiru Olaitan, Ojo Adebola Busola, Oyinloye Babatunji Emmanuel, Imiere Oluwatosin Debbie, Adeyonu Oluwatosin
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J Complement Integr Med. 2017 Mar 17;14(3):/j/jcim.2017.14.issue-3/jcim-2016-0145/jcim-2016-0145.xml. doi: 10.1515/jcim-2016-0145.
Background In West Africa, the fruit, seed, leaf and stem of Blighia sapida K.D. Koenig are commonly used as remedy against a variety of diseases, including diabetes mellitus. This study investigated the ameliorative potential of B. sapida K.D. Koenig stem bark ethanol extract against pancreatic β-cell dysfunction in diabetic rats. Methods Diabetes was induced by intraperitoneal injection of alloxan (65 mg/kg body weight) for 21 days, and orally administered with glibenclamide (5 mg/kg body weight), 50-150 mg/kg body weight of B. sapida stem bark ethanol extract once daily for 21 days. Results The blood glucose levels of rats induced with alloxan were significantly and gradually reduced (p<0.05) in B. sapida stem bark ethanol extract treated animals at the dose of 50-150 mg/kg body weight, and in glibenclamide-treated animals. The significant increase in the lipid peroxidation (malonaldehyde), homeostasis model assessment-insulin resistance scores (HOMA-IR) and decrease in serum insulin, pancreatic β-cell scores as well as antioxidant marker enzymes in untreated diabetic rats compared to normal control rats were reversed by the B. sapida stem bark ethanol extract and glibenclamide. Similarly, histopathological changes in the pancreas were also reversed by the extract and glibenclamide. However, these effects were most prominent in the animals treated with 150 mg/kg body weight of B. sapida bark. Conclusions These findings indicate that B. sapida stem bark possess anti-hyperglycemic activity and exhibits ameliorative potential in managing diabetes.
背景 在西非，西非荔枝（Blighia sapida K.D. Koenig）的果实、种子、叶子和茎通常被用作治疗包括糖尿病在内的多种疾病的药物。本研究调查了西非荔枝茎皮乙醇提取物对糖尿病大鼠胰腺β细胞功能障碍的改善潜力。方法 通过腹腔注射四氧嘧啶（65毫克/千克体重）诱导糖尿病21天，并口服给予格列本脲（5毫克/千克体重）、50 - 150毫克/千克体重的西非荔枝茎皮乙醇提取物，每日一次，持续21天。结果 在给予50 - 150毫克/千克体重的西非荔枝茎皮乙醇提取物处理的动物以及格列本脲处理的动物中，四氧嘧啶诱导的大鼠血糖水平显著且逐渐降低（p<0.05）。与正常对照大鼠相比，未经治疗的糖尿病大鼠脂质过氧化（丙二醛）、稳态模型评估 - 胰岛素抵抗评分（HOMA - IR）显著增加，血清胰岛素、胰腺β细胞评分以及抗氧化标记酶降低，这些变化被西非荔枝茎皮乙醇提取物和格列本脲逆转。同样，提取物和格列本脲也逆转了胰腺的组织病理学变化。然而，这些作用在给予150毫克/千克体重西非荔枝树皮处理的动物中最为显著。结论 这些发现表明西非荔枝茎皮具有降血糖活性，并在糖尿病管理中表现出改善潜力。
