Sinton C M
Research Department, Pharmaceuticals Division, CIBA-GEIGY Corporation, Summit, NJ 07901.
Neuropeptides. 1988 Jul;12(1):29-34. doi: 10.1016/s0143-4179(98)90006-3.
Cholecystokinin (CCK) as the sulfated (CCK-8S) and unsulfated (CCK-8U) octapeptide sequences, and CR 1409 were administered intraventricularly while the action potential (EAP) in the granular cell layer of the hippocampal dentate gyrus evoked by perforant path stimulation was recorded. No consistent effect of the test substances on the amplitude of the EAP was found at doses corresponding to those previously reported to cause an increase in the EAP when administered systemically. Similarly, no effect of CCK on the EAP could be found when the peptide was administered iontophoretically in the granular cell layer. In contrast, iontophoretically applied CCK-8S, CCK-8U and CR 1409 slightly but consistently reduced the slope of the evoked response recorded in the dentate gyrus molecular layer. These results are interpreted as indicating that the CCK receptor on granular cell dendrites is likely to be the central type that is activated by both CCK-8S and CCK-8U, but that any effects of systemically administered CCK on the EAP are probably mediated in the periphery.
以硫酸化（CCK - 8S）和未硫酸化（CCK - 8U）的八肽序列形式给予胆囊收缩素（CCK）以及CR 1409，并通过脑室注射给药，同时记录穿通通路刺激诱发的海马齿状回颗粒细胞层中的动作电位（EAP）。在与先前报道的全身给药时可引起EAP增加的剂量相对应的剂量下，未发现受试物质对EAP幅度有一致的影响。同样，当在颗粒细胞层中通过离子导入法给予CCK时，未发现其对EAP有影响。相反，通过离子导入法应用CCK - 8S、CCK - 8U和CR 1409可轻微但持续地降低在齿状回分子层中记录的诱发反应的斜率。这些结果被解释为表明颗粒细胞树突上的CCK受体可能是由CCK - 8S和CCK - 8U均能激活的中枢型受体，但全身给药的CCK对EAP的任何影响可能在外周介导。
