Tehran University of Medical Sciences, Tehran, Iran.
Folia Med (Plovdiv). 2021 Jun 30;63(3):413-421. doi: 10.3897/folmed.63.e53716.
In fixed orthodontics, formation of white spot lesions, enamel demineralization, and tooth decay around appliances are common complications which mar the outcome.
The aims of this study were the determination of the shear bond strength (SBS) and adhesive remnant index (ARI) of orthodontic adhesive doped with N-GO, as well as the assessment of antimicrobial activities of the modified orthodontic adhesive against Streptococcus mutans.
N-GO was characterized by a scanning electron microscope (SEM), Fourier transformation infrared (FTIR), X-ray diffraction (XRD), and Zeta potential. The SBS and ARI of modified orthodontics adhesive containing different concentrations of N-GO (0, 1, 2, 5, and 10 wt%) were then measured. The influences of adding N-GO on antimicrobial properties of orthodontic adhesive were determined against S. mutans by disc agar diffusion (DAD) testing and biofilm formation inhibition assay.
The SEM, FTIR, XRD, and Zeta potential analysis indicated the successful synthesis of N-GO. Orthodontics adhesive doped with 5 wt% N-GO showed the highest concentration of N-GO and SBS value (21.71±7.45 MPa, p<0.05) simultaneously with no significant differences in adhesive remnant index compared with the control group. SBS in the 1, 2, and 5% N-GO were significantly higher than that in 10% N-GO (p=0.025, p=0.036, p=0.041, respectively). The disinfection ability of the modified orthodontic adhesive doped with N-GO against S. mutans in the DAD and biofilm formation inhibition assays were positively associated with increased in N-GO concentrations (p<0.05). However, the 5 and 10 wt% N-GO showed a statistically significant decrease the CFU/mL of the test microorganisms in biofilm structures (p<0.05).
It could be concluded that 5 wt% of N-GO can be considered as an orthodontic adhesive additive to reduce the microbial count and biofilm with no adverse effect on SBS and ARI.
在固定正畸中，矫治器周围的白垩斑形成、牙釉质脱矿和龋齿是常见的并发症，会影响治疗效果。
本研究旨在测定掺杂纳米氧化石墨烯（N-GO）的正畸胶粘剂的剪切结合强度（SBS）和黏附残留指数（ARI），并评估改良正畸胶粘剂对变形链球菌的抗菌活性。
通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和 Zeta 电位对 N-GO 进行了表征。然后，测量了含有不同浓度 N-GO（0、1、2、5 和 10wt%）的改良正畸胶粘剂的 SBS 和 ARI。通过圆盘琼脂扩散（DAD）试验和生物膜形成抑制试验，确定添加 N-GO 对正畸胶粘剂抗菌性能的影响。
SEM、FTIR、XRD 和 Zeta 电位分析表明成功合成了 N-GO。掺杂 5wt%N-GO 的正畸胶粘剂显示出最高的 N-GO 浓度和 SBS 值（21.71±7.45MPa，p<0.05），同时与对照组相比，ARI 无显著差异。1%、2%和 5%N-GO 的 SBS 显著高于 10%N-GO（p=0.025、p=0.036、p=0.041）。在 DAD 和生物膜形成抑制试验中，掺杂 N-GO 的改良正畸胶粘剂对变形链球菌的消毒能力与 N-GO 浓度的增加呈正相关（p<0.05）。然而，5wt%和 10wt%N-GO 显著降低了生物膜结构中测试微生物的 CFU/mL（p<0.05）。
可以得出结论，5wt%的 N-GO 可以被认为是一种正畸胶粘剂添加剂，以减少微生物计数和生物膜，而不会对 SBS 和 ARI 产生不利影响。
